Overview

  • Product nameAnti-EMAP II antibody (Biotin)
    See all EMAP II primary antibodies
  • Description
    Goat polyclonal to EMAP II (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant human EMAP II

  • General notesab83518 is sterile filtered.

Properties

Applications

Our Abpromise guarantee covers the use of ab83518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Sandwich ELISA
WB
  • Application notesELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human EMAP II.
    sELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human EMAP II.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant human EMAP II is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 34 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNon-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.
    • Involvement in diseaseDefects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3) [MIM:260600]. A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system.
    • Sequence similaritiesContains 1 tRNA-binding domain.
    • Post-translational
      modifications
      Cleaved by caspase-7 in response to apoptosis to produce EMAP-II.
    • Cellular localizationNucleus. Cytoplasm > cytosol. Cytoplasmic vesicle > secretory vesicle. Secreted. Endoplasmic reticulum. Golgi apparatus. Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Also secreted in response to hypoxia and both apoptotic and necrotic cell death.
    • Information by UniProt
    • Database links
    • Alternative names
      • AIMP 1 antibody
      • Aimp1 antibody
      • AIMP1/p43 antibody
      • AIMP1_HUMAN antibody
      • Aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 antibody
      • ARS interacting multifunctional protein 1 antibody
      • EMAP 2 antibody
      • EMAP-2 antibody
      • EMAP-II antibody
      • EMAP2 antibody
      • EMAPII antibody
      • Endothelial monocyte activating polypeptide 2 antibody
      • Endothelial monocyte activating polypeptide antibody
      • Endothelial monocyte-activating polypeptide 2 antibody
      • Endothelial monocyte-activating polypeptide II antibody
      • HLD3 antibody
      • Multisynthase complex auxiliary component p43 antibody
      • Multisynthetase complex auxiliary component p43 antibody
      • OTTHUMP00000219673 antibody
      • p43 antibody
      • Scye 1 antibody
      • Scye1 antibody
      • Scye1, formerly antibody
      • Small inducible cytokine subfamily E member 1 antibody
      • Small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) antibody
      • Small inducible cytokine subfamily E, member 1, formerly antibody
      see all

    References for Anti-EMAP II antibody (Biotin) (ab83518)

    ab83518 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"