Anti-EMAP II antibody - C-terminal (ab175528)

Overview

  • Product name
    Anti-EMAP II antibody - C-terminal
    See all EMAP II primary antibodies
  • Description
    Rabbit polyclonal to EMAP II - C-terminal
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Hamster
  • Immunogen

    Synthetic peptide within Human EMAP II aa 248-277 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q12904

  • Positive control
    • Human lymph node tissue, 293 cells transfected with EMAPII gene.

Properties

Applications

Our Abpromise guarantee covers the use of ab175528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 34 kDa.

Target

  • Function
    Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.
  • Involvement in disease
    Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3) [MIM:260600]. A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system.
  • Sequence similarities
    Contains 1 tRNA-binding domain.
  • Post-translational
    modifications
    Cleaved by caspase-7 in response to apoptosis to produce EMAP-II.
  • Cellular localization
    Nucleus. Cytoplasm > cytosol. Cytoplasmic vesicle > secretory vesicle. Secreted. Endoplasmic reticulum. Golgi apparatus. Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Also secreted in response to hypoxia and both apoptotic and necrotic cell death.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIMP 1 antibody
    • Aimp1 antibody
    • AIMP1/p43 antibody
    • AIMP1_HUMAN antibody
    • Aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 antibody
    • ARS interacting multifunctional protein 1 antibody
    • EMAP 2 antibody
    • EMAP-2 antibody
    • EMAP-II antibody
    • EMAP2 antibody
    • EMAPII antibody
    • Endothelial monocyte activating polypeptide 2 antibody
    • Endothelial monocyte activating polypeptide antibody
    • Endothelial monocyte-activating polypeptide 2 antibody
    • Endothelial monocyte-activating polypeptide II antibody
    • HLD3 antibody
    • Multisynthase complex auxiliary component p43 antibody
    • Multisynthetase complex auxiliary component p43 antibody
    • OTTHUMP00000219673 antibody
    • p43 antibody
    • Scye 1 antibody
    • Scye1 antibody
    • Scye1, formerly antibody
    • Small inducible cytokine subfamily E member 1 antibody
    • Small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) antibody
    • Small inducible cytokine subfamily E, member 1, formerly antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human lymphnode labeling EMAPII with ab175528 at 1/50 followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • All lanes : Anti-EMAP II antibody - C-terminal (ab175528) at 1/1000 dilution

    Lane 1 : 293 cell lysate (non-transfected)
    Lane 2 : 293 cell lysate transiently transfected with the EMAPII gene

    Lysates/proteins at 2 µg per lane.

    Developed using the ECL technique

    Predicted band size : 34 kDa
  • Anti-EMAP II antibody - C-terminal (ab175528) at 1/1000 dilution + 293 cell line lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 34 kDa

References

ab175528 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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