Overview

  • Product name
  • Description
    Mouse polyclonal to EME1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1 - 583 of Human EME1 (AAH16470.1)

  • Positive control
    • HeLa or Jurkat cell lysate. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
  • Sequence similarities
    Belongs to the EME1/MMS4 family.
  • Cellular localization
    Nucleus > nucleolus. Recruited to regions of DNA damage in S-phase cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6820428D13 antibody
    • Crossover junction endonuclease EME1 antibody
    • EC 3.1.22.- antibody
    • EME1 antibody
    • Eme1 essential meiotic endonuclease 1 homolog 1 (S. pombe) antibody
    • EME1, S. pombe, homolog of, 1 antibody
    • EME1_HUMAN antibody
    • essential meiotic endonuclease 1 homolog 1 (S pombe) antibody
    • Essential Meiotic Endonuclease 1 Homolog 1 antibody
    • essential meiotic endonuclease 1 homolog 2 antibody
    • Essential meiotic endonuclease 1, S. pombe, homolog of, 1 antibody
    • FLJ31364 antibody
    • hMMS4 antibody
    • homolog of yeast EME1 endonuclease antibody
    • MGC106543 antibody
    • MMS4 antibody
    • MMS4 homolog antibody
    • MMS4L antibody
    see all

Anti-EME1 antibody images

  • Anti-EME1 antibody (ab88878) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 63 kDa
  • Anti-EME1 antibody (ab88878) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 63 kDa
  • ab88878 at 10 µg/ml staining EME1 in HeLa cells by immunofluorescence.

References for Anti-EME1 antibody (ab88878)

ab88878 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Total protein in input
1000 µg
Username

Abcam user community

Verified customer

Submitted Jul 11 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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