Overview

  • Product name
  • Description
    Mouse monoclonal to Emerin
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-111 of Human Emerin

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab204987 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab54996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 29 kDa.
IHC-P Use a concentration of 1 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.
  • Tissue specificity
    Skeletal muscle, heart, colon, testis, ovary and pancreas.
  • Involvement in disease
    Defects in EMD are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1) [MIM:310300]. A degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects.
  • Sequence similarities
    Contains 1 LEM domain.
  • Post-translational
    modifications
    Found in four different phosphorylated forms, three of which appear to be associated with the cell cycle.
  • Cellular localization
    Nucleus inner membrane. Nucleus outer membrane. Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non-farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify its localization in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EDMD antibody
    • Emd antibody
    • EMD_HUMAN antibody
    • Emerin antibody
    • Emery Dreifuss muscular dystrophy antibody
    • STA antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab54996 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54996, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Emerin antibody (ab54996) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human tonsil.


  • Predicted band size : 29 kDa
    Emerin antibody (ab54996) at 1ug/lane + HeLa cell lysate at 25ug/lane.

References

This product has been referenced in:
  • Zhuang X  et al. Dephosphorylation of barrier-to-autointegration factor by protein phosphatase 4 and its role in cell mitosis. J Biol Chem 289:1119-27 (2014). ICC/IF ; Human . Read more (PubMed: 24265311) »
  • Lomnytska MI  et al. Impact of genomic stability on protein expression in endometrioid endometrial cancer. Br J Cancer 106:1297-305 (2012). WB ; Human . Read more (PubMed: 22415234) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry
Sample
Human Cultured Cells (Breast Cancer)
Specification
Breast Cancer
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 14 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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