Validated using a knockout cell line
Recombinant
RabMAb

Anti-Emerin antibody [EPR11071] (ab156871)

Overview

  • Product name
    Anti-Emerin antibody [EPR11071]
    See all Emerin primary antibodies
  • Description
    Rabbit monoclonal [EPR11071] to Emerin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Emerin aa 150-250. The exact sequence is proprietary.

  • Positive control
    • 293T, Jurkat, A431 and HeLa cell lysates; Human breast tissue, Human thyroid gland carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab156871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
ICC/IF 1/100 - 1/250.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.
    • Tissue specificity
      Skeletal muscle, heart, colon, testis, ovary and pancreas.
    • Involvement in disease
      Defects in EMD are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1) [MIM:310300]. A degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects.
    • Sequence similarities
      Contains 1 LEM domain.
    • Post-translational
      modifications
      Found in four different phosphorylated forms, three of which appear to be associated with the cell cycle.
    • Cellular localization
      Nucleus inner membrane. Nucleus outer membrane. Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non-farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify its localization in the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • EDMD antibody
      • Emd antibody
      • EMD_HUMAN antibody
      • Emerin antibody
      • Emery Dreifuss muscular dystrophy antibody
      • STA antibody
      see all

    Images

    • All lanes : Anti-Emerin antibody [EPR11071] (ab156871) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : EMD (Emerin) knockout HAP1 whole cell lysate
      Lane 3 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 29 kDa



      Lanes 1 - 3: Merged signal (red and green). Green - ab156871 observed at 32 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab156871 was shown to specifically react with Emerin in wild-type HAP1 cells as signal was lost in EMD (Emerin) knockout cells. Wild-type and EMD (Emerin) knockout samples were subjected to SDS-PAGE. Ab156871 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Emerin antibody [EPR11071] (ab156871) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 29 kDa

    • Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Emerin with ab156871 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling Emerin with ab156871 at 1/50 dilution.

    References

    ab156871 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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