Overview

  • Product nameAnti-EMG1 antibody
    See all EMG1 primary antibodies
  • Description
    Rabbit polyclonal to EMG1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: SLETVKVGKT YELLNCDKHK SILLKNGRDP GEARPDITHQ SLLMLMDSPL, corresponding to a region within internal sequence amino acids 51-100 of Human EMG1 (NP_006322.4).

  • Positive control
    • HepG2 cell lysate. Human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab104985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionS-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position (PubMed:20047967). Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes.
  • Involvement in diseaseBowen-Conradi syndrome
  • Sequence similaritiesBelongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.
  • Cellular localizationNucleus, nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 18S rRNA (pseudouridine(1248)-N1)-methyltransferase antibody
    • 18S rRNA Psi1248 methyltransferase antibody
    • C2F antibody
    • C2f protein antibody
    • EMG 1 antibody
    • EMG1 antibody
    • EMG1 N1 specific pseudouridine methyltransferase antibody
    • EMG1 nucleolar protein homolog (S cerevisiae) antibody
    • EMG1 nucleolar protein homolog antibody
    • essential for mitotic growth 1 antibody
    • FLJ60792 antibody
    • Grcc2f antibody
    • NEP1 antibody
    • NEP1_HUMAN antibody
    • Nucleolar protein EMG1 homolog antibody
    • Protein C2f antibody
    • Ribosomal RNA small subunit methyltransferase Nep1 antibody
    • Ribosome biogenesis protein NEP1 antibody
    see all

Anti-EMG1 antibody images

  • Anti-EMG1 antibody (ab104985) at 0.5 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 27 kDa
    12% SDS-PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling EMG1 with ab104985 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells in the renal tubule. Magnification: 400X.

References for Anti-EMG1 antibody (ab104985)

ab104985 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"