This antibody gave a positive signal in the following tissue lysates: Human Small Intestine; Human Cervix; Human Fetal Heart; Human Fetal Kidney.
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1/250. Detects a band of approximately 130 kDa (predicted molecular weight: 106 kDa).
May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity.
Distributed in tissues where resilience and elastic recoil are prominent. Highest levels in the adult small intestine, aorta, lung, uterus, and appendix and in the fetal spleen, kidney, lung, and heart; intermediate expression was detected in adult liver, ovary, colon, stomach, lymph node and spleen; adult heart, bladder, prostate, adrenal gland, mammary gland, placenta and kidney showed low expression whereas a series of other adult tissues, including skeletal muscle and different regions of adult brain show no expression.
Elastin microfibril interface located protein 1 antibody
Elastin microfibril interface located protein antibody
Elastin microfibril interface-located protein 1 antibody
Elastin microfibril interfacer 1 antibody
EMILIN 1 antibody
Western blot - Anti-EMILIN1 antibody (ab125860)
All lanes : Anti-EMILIN1 antibody (ab125860) at 1/250 dilution
Lane 1 : Human small intestine tissue lysate - total protein (ab29276) Lane 2 : Human cervix normal tissue lysate - total protein (ab29226) Lane 3 : Kidney (Human) Tissue Lysate - fetal normal tissue (ab30204) Lane 4 : Heart (Human) Whole Cell Lysate - fetal normal tissue
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
EMILIN1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125860 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.