Overview

  • Product name
  • Description
    Rabbit polyclonal to EMILIN1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human EMILIN1.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human Small Intestine; Human Cervix; Human Fetal Heart; Human Fetal Kidney.

Properties

Applications

Our Abpromise guarantee covers the use of ab125860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 130 kDa (predicted molecular weight: 106 kDa).

Target

  • Function
    May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity.
  • Tissue specificity
    Distributed in tissues where resilience and elastic recoil are prominent. Highest levels in the adult small intestine, aorta, lung, uterus, and appendix and in the fetal spleen, kidney, lung, and heart; intermediate expression was detected in adult liver, ovary, colon, stomach, lymph node and spleen; adult heart, bladder, prostate, adrenal gland, mammary gland, placenta and kidney showed low expression whereas a series of other adult tissues, including skeletal muscle and different regions of adult brain show no expression.
  • Sequence similarities
    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
    Contains 1 EMI domain.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix. Found mainly at the interface between amorphous elastin and microfibrils.
  • Information by UniProt
  • Database links
  • Alternative names
    • Elastin microfibril interface located protein 1 antibody
    • Elastin microfibril interface located protein antibody
    • Elastin microfibril interface-located protein 1 antibody
    • Elastin microfibril interfacer 1 antibody
    • EMIL1_HUMAN antibody
    • EMILIN 1 antibody
    • EMILIN antibody
    • EMILIN-1 antibody
    • Emilin1 antibody
    • gp115 antibody
    see all

Images

  • All lanes : Anti-EMILIN1 antibody (ab125860) at 1/250 dilution

    Lane 1 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 2 : Human cervix normal tissue lysate - total protein (ab29226)
    Lane 3 : Kidney (Human) Tissue Lysate - fetal normal tissue (ab30204)
    Lane 4 : Heart (Human) Whole Cell Lysate - fetal normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 106 kDa
    Observed band size: 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 160 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds


    EMILIN1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125860 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab125860 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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