Recombinant Anti-EMILIN1 antibody [EPR14678] - N-terminal (ab185953)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14678] to EMILIN1 - N-terminal
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-EMILIN1 antibody [EPR14678] - N-terminal
See all EMILIN1 primary antibodies -
Description
Rabbit monoclonal [EPR14678] to EMILIN1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human uterus, fetal kidney and colon cancer tissue lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14678 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/30 - 1/50.
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WB |
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 107 kDa).
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Notes |
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IP
1/30 - 1/50. |
WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 107 kDa). |
Target
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Function
May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity. -
Tissue specificity
Distributed in tissues where resilience and elastic recoil are prominent. Highest levels in the adult small intestine, aorta, lung, uterus, and appendix and in the fetal spleen, kidney, lung, and heart; intermediate expression was detected in adult liver, ovary, colon, stomach, lymph node and spleen; adult heart, bladder, prostate, adrenal gland, mammary gland, placenta and kidney showed low expression whereas a series of other adult tissues, including skeletal muscle and different regions of adult brain show no expression. -
Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain.
Contains 1 EMI domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. Found mainly at the interface between amorphous elastin and microfibrils. - Information by UniProt
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Database links
- Entrez Gene: 11117 Human
- Omim: 130660 Human
- SwissProt: Q9Y6C2 Human
- Unigene: 63348 Human
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Alternative names
- Elastin microfibril interface located protein 1 antibody
- Elastin microfibril interface located protein antibody
- Elastin microfibril interface-located protein 1 antibody
see all
Images
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All lanes : Anti-EMILIN1 antibody [EPR14678] - N-terminal (ab185953) at 1/50000 dilution
Lane 1 : Human uterus tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : Human colon cancer tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 107 kDa -
Western blot analysis of immunoprecipitation pellet from Human fetal kidney tissue lysate labeling EMILIN1 with ab185953 at a 1/50 dilution. Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, at a 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab185953 has been referenced in 1 publication.
- Rabajdová M et al. Detection of Pathological Changes in the Aorta during Thoracic Aortic Aneurysm Progression on Molecular Level. Dis Markers 2017:9185934 (2017). PubMed: 29158612