Overview

  • Product nameAnti-EML3 antibody
  • Description
    Rabbit polyclonal to EML3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A synthetic peptide corresponding to a region within the N terminal amino acids 180-229 (LLVRSGSTES RGGKDPLSSP GGPGSRRSNY NLEGISVKMF LRGRPITMYI) of human EML3 (NP_694997).

  • Positive control
    • Human placenta lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 95 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceEML3 is a nuclear microtubule binding protein required for the correct alignment of chromosomes in metaphase. EML3 may modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • Echinoderm microtubule associated protein like 3 antibody
    • ELP 95 antibody
    • EMAP 3 antibody
    • EML 3 antibody
    • FLJ 35827 antibody
    • MGC 111422 antibody
    see all

Anti-EML3 antibody images

  • Anti-EML3 antibody (ab83821) at 1 µg/ml + human placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 95 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)

References for Anti-EML3 antibody (ab83821)

ab83821 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"