The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 95 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceEML3 is a nuclear microtubule binding protein required for the correct alignment of chromosomes in metaphase. EML3 may modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.