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Rabbit polyclonal to EML4
Predicted to work with:
Synthetic peptide, corresponding to a region within amino acids 931-981 of Human EML4 (NP_061936.2)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 109 kDa.
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.
Belongs to the WD repeat EMAP family.
Contains 9 WD repeats.
Strongly overexpressed during mitosis.
Cytoplasm > cytoskeleton.
Information by UniProt
Echinoderm microtubule associated protein like 4 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EML4 antibody (ab85834)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling EML4 with ab85834 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-EML4 antibody (ab85834)
All lanes :
Anti-EML4 antibody (ab85834) at 0.1 µg/ml
Lane 1 :
Whole cell lysate from HeLa cells at 50 µg
Lane 2 :
Whole cell lysate from HeLa cells at 15 µg
Lane 3 :
Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size:
Observed band size:
120 kDa (
why is the actual band size different from the predicted?
Additional bands at:
31 kDa, 60 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Anti-EML4 antibody (ab85834)
Immunoprecipitation/ Western Blot of ab85834
Lane 1: ab85834 at 3µg/mg whole cell lysate.Lane 2: Control IgG. ab85834 at 0.4µg/ml for WB.Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"