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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 109 kDa.
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Function May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.
Sequence similarities Belongs to the WD repeat EMAP family. Contains 9 WD repeats.
Developmental stage Strongly overexpressed during mitosis.
Cellular localization Cytoplasm > cytoskeleton.
Information by UniProt
Echinoderm microtubule associated protein like 4 antibody
Anti-EML4 antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EML4 antibody (ab85834)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling EML4 with ab85834 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - EML4 antibody (ab85834)
All lanes : Anti-EML4 antibody (ab85834) at 0.1 µg/ml Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Developed using the ECL technique Predicted band size : 109 kDa Observed band size : 120 kDa ( why is the actual band size different from the predicted?) Additional bands at : 31 kDa,60 kDa,72 kDa. We are unsure as to the identity of these extra bands. Exposure time : 3 minutes
Immunoprecipitation - EML4 antibody (ab85834)
Immunoprecipitation/ Western Blot of ab85834 Lane 1: ab85834 at 3µg/mg whole cell lysate.Lane 2: Control IgG. ab85834 at 0.4µg/ml for WB.Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 30 seconds.
References for Anti-EML4 antibody (ab85834)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"