Overview

  • Product name
  • Description
    Rabbit polyclonal to EML4
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 931-981 of Human EML4 (NP_061936.2)

  • Positive control
    • HeLa whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 109 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.
  • Sequence similarities
    Belongs to the WD repeat EMAP family.
    Contains 9 WD repeats.
  • Developmental stage
    Strongly overexpressed during mitosis.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • EML4 antibody
    • C2orf2 antibody
    • Echinoderm microtubule associated protein like 4 antibody
    • Echinoderm microtubule-associated protein-like 4 antibody
    • ELP 120 antibody
    • ELP120 antibody
    • EMAL4_HUMAN antibody
    • EMAP 4 antibody
    • EMAP-4 antibody
    • EMAP4 antibody
    • EML 4 antibody
    • eml4 antibody
    • Restrictedly overexpressed proliferation associated protein antibody
    • Restrictedly overexpressed proliferation-associated protein antibody
    • Ropp 120 antibody
    • ROPP120 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling EML4 with ab85834 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-EML4 antibody (ab85834) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 109 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 31 kDa,60 kDa,72 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Immunoprecipitation/ Western Blot of ab85834
    Lane 1: ab85834 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    ab85834 at 0.4µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Chemiluminescence with an exposure time of 30 seconds.

References

ab85834 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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