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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab86062 was affinity purified using an epitope specific to EML4 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 109 kDa).
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Function May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.
Sequence similarities Belongs to the WD repeat EMAP family. Contains 9 WD repeats.
Developmental stage Strongly overexpressed during mitosis.
Cellular localization Cytoplasm > cytoskeleton.
Information by UniProt
Echinoderm microtubule associated protein like 4 antibody
Anti-EML4 antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EML4 antibody (ab86062)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lin plastica stomach cancer tissue labelling EML4 with ab86062 at 1/200 (1µg/ml). Detection: DAB.
Western blot - EML4 antibody (ab86062)
All lanes : Anti-EML4 antibody (ab86062) at 1/0.04 dilution Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Predicted band size : 109 kDa Observed band size : 120 kDa ( why is the actual band size different from the predicted?) Exposure time : 30 seconds
Immunoprecipitation - EML4 antibody (ab86062)
Detection of EML4 by Western Blot of Immunprecipitate.
ab86062 at 0.4µg/ml staining EML4 in HeLa whole cell lysate immunoprecipitated using ab86062 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
References for Anti-EML4 antibody (ab86062)
has not yet been referenced specifically in any publications.
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