Overview

  • Product nameAnti-EMR2 antibody
    See all EMR2 primary antibodies
  • Description
    Rabbit polyclonal to EMR2
  • Specificityab75190 detects endogenous levels of total EMR2 protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal region of human EMR2

  • Positive control
    • HeLa cell extract

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab75190 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 91 kDa.
ELISA 1/5000.

Target

  • FunctionReceptor probably involved in cell attachment.
  • Tissue specificityExpression is restricted to myeloid cells. Highest expression was found in peripheral blood leukocytes, followed by spleen and lymph nodes, with intermediate to low levels in thymus, bone marrow, fetal liver, placenta, and lung, and no expression in heart, brain, skeletal muscle, kidney, or pancreas. Expression is also detected in monocyte/macrophage and Jurkat cell lines but not in other cell lines tested.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
    Contains 5 EGF-like domains.
    Contains 1 GPS domain.
  • DomainThe GPS domain is necessary, but not sufficient for receptor cleavage, which require the entire extracellular stalk.
    Binding to chondroitin sulfate is mediated by the fourth EGF domain.
  • Post-translational
    modifications
    Autoproteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane beta subunit.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 312 antibody
    • CD 312 antigen antibody
    • CD312 antibody
    • CD312 antigen antibody
    • Egf like module containing mucin like hormone receptor like 2 antibody
    • Egf like module containing mucin like hormone receptor like sequence 2 antibody
    • EGF like module EMR2 antibody
    • EGF-like module receptor 2 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 2 antibody
    • EMR2 antibody
    • EMR2_HUMAN antibody
    see all

Anti-EMR2 antibody images

  • All lanes : Anti-EMR2 antibody (ab75190) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with immunising peptide at 10 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 91 kDa
    Observed band size : 91 kDa

References for Anti-EMR2 antibody (ab75190)

ab75190 has not yet been referenced specifically in any publications.

Product Wall

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Thank you for sending me this further information. It has helped tremendously in understanding the issues that you have been facing.

I agree with your assessment that these bands are either non-specific or isoforms 4 and 5. As we cannot be s...

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I am sorry about the issues you have been having. Thank you for bringing this to our attention. As this product should not be recognizing the other forms of EMR2 it appears that you may be experiencing non specific background issues. I may be able to h...

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I have had to opportunity to speak with the lab further regarding this. The immunogen sequence for this product is:XXX which spans aaXXX-XXX (SwissProt: Q9UHX3Human). This sequence is within the excellular region and is not in isoforms 4 and 5. Are ...

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Thank you for contacting us.

I have had the opportunity to speak with the labs in regard to your questions about these product.

The immunogen sequence for each product is proprietary but the labs have run alignments and checked ...

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