Overview

  • Product name
    Anti-EMSY antibody [Y474]
    See all EMSY primary antibodies
  • Description
    Rabbit monoclonal [Y474] to EMSY
  • Specificity
    ab32329 recognises EMSY.
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human EMSY aa 1-100 (C terminal).
    Database link: Q7Z589
    (Peptide available as ab174721)

  • Epitope
    ab32329 reacts with an epitope located in the C terminal region if EMSY.
  • Positive control
    • MCF7 cells NIH/3T3 (human neuroblastoma) whole cell lysate C6 (rat glioma) whole cell lysate HeLa (human cervix adenocarcinoma) whole cell lysate
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab32329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 141 kDa.
IP 1/30.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    Images

    • All lanes : Anti-EMSY antibody [Y474] (ab32329) at 1/10000 dilution

      Lane 1 : NIH/3T3 (mouse embryo) whole cell lysate
      Lane 2 : C6 (rat glioma) whole cell lysate
      Lane 3 : Rat Ovary

      Lysates/proteins at 20 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 141 kDa
      Observed band size : 141 kDa

      Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-EMSY antibody [Y474] (ab32329) at 1/10000 dilution

      Lane 1 : MCF-7 (human breast carcinoma) whole cell lysate
      Lane 2 : La (human cervix adenocarcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 141 kDa
      Observed band size : 141 kDa

      Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

       

    • Anti-EMSY antibody [Y474] (ab32329) at 1/1000 dilution + MCF7 cell lysate

      Predicted band size : 141 kDa
      Observed band size : 141 kDa
    • Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

    References

    This product has been referenced in:
    • Tian J  et al. Blocking the PI3K/AKT pathway enhances mammalian reovirus replication by repressing IFN-stimulated genes. Front Microbiol 6:886 (2015). Read more (PubMed: 26388843) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you for your enquiry. Rabbit monoclonal [Y474] to EMSY (ab32329) was raised using a synthetic peptide corresponding to residues near the C-terminal region of human EMSY isoform 1. The exact sequence is as follows: CGNVGQKKAEESPAEI I hope t...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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