Anti-Emx1 antibody (ab125443)

Overview

  • Product name
  • Description
    Rabbit polyclonal to Emx1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Guinea pig, Cow, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 127-176 (PWVLRNRFFG HRFQASDVPQ DGLLLHGPFA RKPKRIRTAF SPSQLLRLER) of Mouse Emx1 (NP_034261).

  • Positive control
    • Mouse pancreas lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab125443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
  • Tissue specificity
    Cerebral cortex.
  • Sequence similarities
    Belongs to the EMX homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Empty spiracles homeobox 1 antibody
    • Empty spiracles homolog 1 antibody
    • empty spiracles homolog 1 Drosophila antibody
    • Empty spiracles like protein 1 antibody
    • Empty spiracles-like protein 1 antibody
    • EMX1 antibody
    • EMX1_HUMAN antibody
    • Homeobox protein EMX1 antibody
    see all

Anti-Emx1 antibody images

  • Anti-Emx1 antibody (ab125443) at 1 µg/ml + Mouse pancreas lysate at 10 µg

    Predicted band size : 28 kDa

References for Anti-Emx1 antibody (ab125443)

ab125443 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain hemisphere coronal sections)
Specification
Brain hemisphere coronal sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - Triton X-100 0.3%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Username

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Verified customer

Submitted Jul 24 2012

DISCOUNT CODE: 10***
Expiration date: October 15, 2012
This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab125443 in IHC and include this code in the “Additional Com...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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