This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Emx1 antibody (ab32925)

Overview

Properties

Applications

  • Application notesThis antibody gave a positive result in ELISA against the immunizing peptide (ab32995).

    IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

  • Target

    • FunctionTranscription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
    • Tissue specificityCerebral cortex.
    • Sequence similaritiesBelongs to the EMX homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Empty spiracles homeobox 1 antibody
      • Empty spiracles homolog 1 antibody
      • empty spiracles homolog 1 Drosophila antibody
      • Empty spiracles like protein 1 antibody
      • Empty spiracles-like protein 1 antibody
      • EMX1 antibody
      • EMX1_HUMAN antibody
      • Homeobox protein EMX1 antibody
      see all

    Anti-Emx1 antibody images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • Emx1 expression in adult rat cerebellum, using ab32925 at 1/200 dilution.
    • Emx1 expression in rat striatum, using ab32925 at 1/200 dilution.
    • Emx1 staining of the rat primary motor cortex using ab32925 at 1/200.

    References for Anti-Emx1 antibody (ab32925)

    ab32925 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry and for your patience. It has taken some time to discuss this with our source for this antibody.

    It is unfortunately difficult to say what the lighter brown staining is exactly in the IHC image provided for ab23340...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Mouse E17 forebrain)
    Specification Mouse E17 forebrain
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Permeabilization Yes - 0.025% TX100
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 21 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"