This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Emx1 antibody (ab32925)

Overview

Properties

Applications

  • Application notes
    This antibody gave a positive result in ELISA against the immunizing peptide (ab32995).

    IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

  • Target

    • Function
      Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
    • Tissue specificity
      Cerebral cortex.
    • Sequence similarities
      Belongs to the EMX homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Empty spiracles homeobox 1 antibody
      • Empty spiracles homolog 1 antibody
      • empty spiracles homolog 1 Drosophila antibody
      • Empty spiracles like protein 1 antibody
      • Empty spiracles-like protein 1 antibody
      • EMX1 antibody
      • EMX1_HUMAN antibody
      • Homeobox protein EMX1 antibody
      see all

    Images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • Emx1 expression in adult rat cerebellum, using ab32925 at 1/200 dilution.
    • Emx1 expression in rat striatum, using ab32925 at 1/200 dilution.
    • Emx1 staining of the rat primary motor cortex using ab32925 at 1/200.

    References

    ab32925 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your enquiry and for your patience. It has taken some time to discuss this with our source for this antibody.

    It is unfortunately difficult to say what the lighter brown staining is exactly in the IHC image provided for ab23340...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mouse E17 forebrain)
    Specification
    Mouse E17 forebrain
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Permeabilization
    Yes - 0.025% TX100
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

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    Verified customer

    Submitted Aug 21 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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