Overview

  • Product name
  • Description
    Rabbit polyclonal to EMX2
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide within Human EMX2 aa 118-147 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. NP_004089.1.
    Database link: Q04743

  • Positive control
    • HL60 cells. HL60 cell line lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99.9% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab174897 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 28 kDa.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
  • Tissue specificity
    Cerebral cortex.
  • Involvement in disease
    Defects in EMX2 are the cause of schizencephaly (SCHZC) [MIM:269160]. Schizencephaly is an extremely rare human congenital disorder characterized by a full-thickness cleft within the cerebral hemispheres. These clefts are lined with gray matter and most commonly involve the parasylvian regions. Large portions of the cerebral hemispheres may be absent and replaced by cerebro-spinal fluid.
  • Sequence similarities
    Belongs to the EMX homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Empty spiracles homeobox 2 antibody
    • Empty spiracles homolog 2 (Drosophila) antibody
    • Empty spiracles homolog 2 antibody
    • Empty spiracles like protein 2 antibody
    • Empty spiracles-like protein 2 antibody
    • EMX 2 antibody
    • EMX2 antibody
    • EMX2_HUMAN antibody
    • Homeobox protein EMX 2 antibody
    • Homeobox protein EMX2 antibody
    see all

Images

  • Anti-EMX2 antibody (ab174897) at 1/100 dilution + HL60 cell lysate at 35 µg

    Predicted band size : 28 kDa
  • Flow cytometric analysis of HL-60 cells (right histogram) labeling EMX2 with ab174897 at 1/10 dilution compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab174897 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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