The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Direct ELISA: 1/19000.
WB: 1/1000. Predicted molecular weight: 28 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
Involvement in disease
Defects in EMX2 are the cause of schizencephaly (SCHZC) [MIM:269160]. Schizencephaly is an extremely rare human congenital disorder characterized by a full-thickness cleft within the cerebral hemispheres. These clefts are lined with gray matter and most commonly involve the parasylvian regions. Large portions of the cerebral hemispheres may be absent and replaced by cerebro-spinal fluid.
Belongs to the EMX homeobox family. Contains 1 homeobox DNA-binding domain.
Lane 1 : Anti-EMX2 antibody (ab86004) at 1/1000 dilution (in 5% non fat milk PBST solution incubated for 60 minutes) Lane 2 : Anti-EMX2 antibody (ab86004) (in 5% non fat milk PBST solution incubated for 60 minutes)
Lane 1 : uninduced culture of E. coli (plus anti thio at 1/5000 dilution) Lane 2 : induced culture of E. coli (plus anti thio at 1/5000 dilution)
Secondary All lanes : anti-Rabbit IgG HRP conjugate incubated for 60 minutes at 1/1000 dilution