Overview

  • Product name
  • Description
    Rabbit polyclonal to EMX2
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Mouse, Human
  • Immunogen

    Three synthetic peptides derived from human EMX2, conjugated to KLH.

  • Positive control
    • An anti thio tested on an induced culture of E.coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab86004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Direct ELISA: 1/19000.
    WB: 1/1000. Predicted molecular weight: 28 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
    • Tissue specificity
      Cerebral cortex.
    • Involvement in disease
      Defects in EMX2 are the cause of schizencephaly (SCHZC) [MIM:269160]. Schizencephaly is an extremely rare human congenital disorder characterized by a full-thickness cleft within the cerebral hemispheres. These clefts are lined with gray matter and most commonly involve the parasylvian regions. Large portions of the cerebral hemispheres may be absent and replaced by cerebro-spinal fluid.
    • Sequence similarities
      Belongs to the EMX homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Empty spiracles homeobox 2 antibody
      • Empty spiracles homolog 2 (Drosophila) antibody
      • Empty spiracles homolog 2 antibody
      • Empty spiracles like protein 2 antibody
      • Empty spiracles-like protein 2 antibody
      • EMX 2 antibody
      • EMX2 antibody
      • EMX2_HUMAN antibody
      • Homeobox protein EMX 2 antibody
      • Homeobox protein EMX2 antibody
      see all

    Images

    • Lane 1 : Anti-EMX2 antibody (ab86004) at 1/1000 dilution (in 5% non fat milk PBST solution incubated for 60 minutes)
      Lane 2 : Anti-EMX2 antibody (ab86004) (in 5% non fat milk PBST solution incubated for 60 minutes)

      Lane 1 : uninduced culture of E. coli (plus anti thio at 1/5000 dilution)
      Lane 2 : induced culture of E. coli (plus anti thio at 1/5000 dilution)

      Secondary
      anti-Rabbit IgG HRP conjugate incubated for 60 minutes at 1/1000 dilution

      Predicted band size : 28 kDa
      Observed band size : 42 kDa (why is the actual band size different from the predicted?)

    References

    ab86004 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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