Overview

  • Product name
  • Description
    Rabbit polyclonal to EMX2
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Chicken, Cow, Dog, Human, Pig, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse EMX2.

    (Peptide available as ab109642.)

  • Positive control
    • This antibody gave a positive signal in both Mouse brain and hippocampus tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab94713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
  • Tissue specificity
    Cerebral cortex.
  • Involvement in disease
    Defects in EMX2 are the cause of schizencephaly (SCHZC) [MIM:269160]. Schizencephaly is an extremely rare human congenital disorder characterized by a full-thickness cleft within the cerebral hemispheres. These clefts are lined with gray matter and most commonly involve the parasylvian regions. Large portions of the cerebral hemispheres may be absent and replaced by cerebro-spinal fluid.
  • Sequence similarities
    Belongs to the EMX homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Empty spiracles homeobox 2 antibody
    • Empty spiracles homolog 2 (Drosophila) antibody
    • Empty spiracles homolog 2 antibody
    • Empty spiracles like protein 2 antibody
    • Empty spiracles-like protein 2 antibody
    • EMX 2 antibody
    • EMX2 antibody
    • EMX2_HUMAN antibody
    • Homeobox protein EMX 2 antibody
    • Homeobox protein EMX2 antibody
    see all

Images

  • All lanes : Anti-EMX2 antibody (ab94713) at 1 µg/ml

    Lane 1 : Mouse Hippocampus Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 300 kDa,74 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References

ab94713 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (BRAIN)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
40 µg
Specification
BRAIN
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Kyungjoo Seong

Verified customer

Submitted Nov 30 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Human Cell (E14 mouse ES cells transduced with lentiviral EMX2)
Specification
E14 mouse ES cells transduced with lentiviral EMX2
Permeabilization
Yes - 10min Triton X-100 RT
Fixative
Paraformaldehyde
Username

Mr. Albrecht

Verified customer

Submitted Dec 08 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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