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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab83693 is purified by a peptide affinity chromatography method.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/312500.
Sequence similarities Belongs to the engrailed homeobox family. Contains 1 homeobox DNA-binding domain.
Cellular localization Nucleus.
Information by UniProt
En 1 antibody
Engrailed 1 antibody
Anti-EN1 / Engrailed 1 antibody images
Western blot - EN1 / Engrailed 1 antibody (ab83693)
Anti-EN1 / Engrailed 1 antibody (ab83693) at 1 µg/ml + HepG2 cell lysate at 10 µg Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size : 40 kDa Observed band size : 37 kDa ( why is the actual band size different from the predicted?) Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.
References for Anti-EN1 / Engrailed 1 antibody (ab83693)
This product has been referenced in:
et al. Novel role of Engrailed 1 as a prosurvival transcription factor in basal-like breast cancer and engineering of interference peptides block its oncogenic function. Oncogene N/A:N/A (2013).
Read more (PubMed: 24141779) »
See 1 Publication
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"