Overview

  • Product name
    Anti-EN1 / Engrailed 1 antibody
    See all EN1 / Engrailed 1 primary antibodies
  • Description
    Rabbit polyclonal to EN1 / Engrailed 1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Zebrafish
  • Immunogen

    Synthetic peptide designed within residues: LMGSANGGPV VKTDSQQPLV WPAWVYCTRY SDRPSSGPRT RKLKKKKNEK , corresponding to C terminal amino acids 253-302 of Human EN1/ Engailed 1 (NP_001417)

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

Images

  • Anti-EN1 / Engrailed 1 antibody (ab83693) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Beltran AS  et al. Novel role of Engrailed 1 as a prosurvival transcription factor in basal-like breast cancer and engineering of interference peptides block its oncogenic function. Oncogene N/A:N/A (2013). Read more (PubMed: 24141779) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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