Anti-EN1 / Engrailed 1 antibody - Aminoterminal end (ab70993)

Overview

  • Product nameAnti-EN1 / Engrailed 1 antibody - Aminoterminal end
    See all EN1 / Engrailed 1 primary antibodies
  • Description
    Rabbit polyclonal to EN1 / Engrailed 1 - Aminoterminal end
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the N-terminal region of human EN1/ Engrailed 1

  • Positive control
    • NCI-H460 cell line lysates, human lung carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab70993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ICC/IF 1/10 - 1/50.
IHC-P 1/10 - 1/50.

Target

  • Sequence similaritiesBelongs to the engrailed homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • En 1 antibody
    • EN1 antibody
    • Engrailed 1 antibody
    • Engrailed antibody
    • Engrailed homeobox 1 antibody
    • Engrailed homolog 1 antibody
    • HME1_HUMAN antibody
    • Homeobox protein en-1 antibody
    • Homeobox protein engrailed 1 antibody
    • Homeobox protein engrailed-1 antibody
    • Hu-En-1 antibody
    • Mo-en.1 antibody
    • OTTHUMP00000162091 antibody
    see all

Anti-EN1 / Engrailed 1 antibody - Aminoterminal end images

  • Anti-EN1 / Engrailed 1 antibody - Aminoterminal end (ab70993) at 1/60 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 55 kDa,<28 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with 1/50 ab70993. A peroxidase-conjugated secondary antibody was used, followed by DAB staining.
  • Fluorescent image of U251 cell stained with ab70993. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab70993 (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/mL, 1 h at 37℃).

References for Anti-EN1 / Engrailed 1 antibody - Aminoterminal end (ab70993)

This product has been referenced in:
  • Wang F  et al. Comparative N-glycoproteomic and phosphoproteomic profiling of human placental plasma membrane between normal and preeclampsia pregnancies with high-resolution mass spectrometry. PLoS One 8:e80480 (2013). IHC-P ; Human . Read more (PubMed: 24260401) »

See 1 Publication for this product

Product Wall

Arthur,

This is probably the same list that you came up with, for Pax6 and synaptophysin we have abs that match the requirments:

Pax6
http://www.abcam.com/PAX6-antibody-Stem-Cell-Marker-ab5790.html

Synaptophysin
http:...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"