KLH conjugated synthetic peptide selected from the N-terminal region of human EN1/ Engrailed 1
Our Abpromise guarantee covers the use of ab70993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).|
|ICC/IF||1/10 - 1/50.|
|IHC-P||1/10 - 1/50.|
Fluorescent image of U251 cell stained with ab70993. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab70993 (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/mL, 1 h at 37℃).