Overview

  • Product name
  • Description
    Rabbit polyclonal to EN2
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide derived from residues 284-333 (C terminal) of human EN2.

    .

  • Positive control
    • WB: Fetal cerebellum lysate. IHC-P: Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab28731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2500.
WB Use a concentration of 0.05 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Relevance
    EN1 and EN2 encode homeodomain containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. EN2 may play a role in susceptibility to autism spectrum disorders.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • AUTS 1 antibody
    • AUTS1 antibody
    • EN 2 antibody
    • Engrailed 2 antibody
    • Engrailed homeo box 2 antibody
    • Engrailed homeobox 2 antibody
    • Engrailed homolog 2 antibody
    • Engrailed2 antibody
    • Homeobox protein engrailed 2 antibody
    • Homeobox protein engrailed2 antibody
    • Hu En 2 antibody
    • HuEn2 antibody
    • MabEn antibody
    see all

Images

  • Anti-EN2 antibody (ab28731) at 0.05 µg/ml + fetal cerebellum lysate at 10 µg

    Secondary
    HRP conjugated anti rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34,92 kDa (why is the actual band size different from the predicted?)
  • Ab28731, at a dilution of 4.0-8.0 µg/ml, staining EN2 in paraffin embedded human kidney tissues by Immunohistochemistry.
    Cells with positive label: epithelial cells of renal tubule (indicated with arrows).
    Magnification: 400X.
  • Anti-EN2 antibody (ab28731) at 1/1000 dilution + Human renal tumor nuclear cell lysate at 30 µg

    Secondary
    Alkaline phosphate conjugated goat anti-rabbit polyclonal antibody
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 34 kDa


    Exposure time : 40 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References

This product has been referenced in:
  • Guan R  et al. A novel protein is lower expressed in renal cell carcinoma. Int J Mol Sci 15:7398-408 (2014). WB, ELISA, IHC . Read more (PubMed: 24786097) »
  • James SJ  et al. Complex epigenetic regulation of Engrailed-2 (EN-2) homeobox gene in the autism cerebellum. Transl Psychiatry 3:e232 (2013). WB ; Human . Read more (PubMed: 23423141) »

See all 3 Publications for this product

Customer reviews and Q&As



There has been some error on our datsheet on the abcam website. The two catalogue numbers refer to the same antibody. You can order using any one of them.

This antibody was raised against a short peptide conjugated to KLH as the carrier protein.

Thank you for your enquiry. The antibody was raised against a peptide conjugated to KLH, keyhole limpet hemocyanin. Please let us know if you have any other questions.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (prostate tumour)
Specification
prostate tumour
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Abcam user community

Verified customer

Submitted Mar 16 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (renal tumour)
Loading amount
30 µg
Specification
renal tumour
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
Username

Abcam user community

Verified customer

Submitted Dec 13 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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