Overview

  • Product nameAnti-ENAH antibody
    See all ENAH primary antibodies
  • Description
    Rabbit polyclonal to ENAH
  • SpecificityDetects endogenous levels of total ENAH protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthesized peptide derived from internal of human ENAH.

  • Positive control
    • Extracts from HepG2 cells and human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab92849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notesELISA: 1/10,000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 67 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEna/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes.
    • Tissue specificityExpressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions.
    • Sequence similaritiesBelongs to the Ena/VASP family.
      Contains 1 WH1 domain.
    • DomainThe EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
    • Post-translational
      modifications
      NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > filopodium. Cell junction > synapse. Cell junction > focal adhesion. Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses.
    • Information by UniProt
    • Database links
    • Alternative names
      • ENA antibody
      • Enah antibody
      • ENAH_HUMAN antibody
      • MENA antibody
      • NDPP1 antibody
      • Protein enabled homolog antibody
      see all

    Anti-ENAH antibody images

    • All lanes : Anti-ENAH antibody (ab92849) at 1/500 dilution

      Lane 1 : Extracts from HepG2 cells
      Lane 2 : Extracts from HepG2 cells with peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 67 kDa
    • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ab92849 at 1/50-1/100 dilution with (+) and without (-) immunising peptide.

    References for Anti-ENAH antibody (ab92849)

    ab92849 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (neuron)
    Permeabilization Yes - 0.2%Triton
    Specification neuron
    Blocking step Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative Formaldehyde
    Username

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    Verified customer

    Submitted Oct 13 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Monkey Cell (COS7)
    Permeabilization Yes - 0.2%Triton
    Specification COS7
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Oct 07 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (brain)
    Loading amount 40 µg
    Specification brain
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Jun 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"