• Product nameAnti-ENAH antibody
    See all ENAH primary antibodies
  • Description
    Rabbit polyclonal to ENAH
  • SpecificityDetects endogenous levels of total ENAH protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthesized peptide derived from internal of human ENAH.

  • Positive control
    • Extracts from HepG2 cells and human breast carcinoma tissue



Our Abpromise guarantee covers the use of ab92849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/10,000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 67 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEna/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes.
    • Tissue specificityExpressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions.
    • Sequence similaritiesBelongs to the Ena/VASP family.
      Contains 1 WH1 domain.
    • DomainThe EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
    • Post-translational
      NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > filopodium. Cell junction > synapse. Cell junction > focal adhesion. Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses.
    • Information by UniProt
    • Database links
    • Alternative names
      • ENA antibody
      • Enah antibody
      • ENAH_HUMAN antibody
      • MENA antibody
      • NDPP1 antibody
      • Protein enabled homolog antibody
      see all

    Anti-ENAH antibody images

    • All lanes : Anti-ENAH antibody (ab92849) at 1/500 dilution

      Lane 1 : Extracts from HepG2 cells
      Lane 2 : Extracts from HepG2 cells with peptide at 10 µg

      Lysates/proteins at 30 µg per lane.

      Predicted band size : 67 kDa
    • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ab92849 at 1/50-1/100 dilution with (+) and without (-) immunising peptide.

    References for Anti-ENAH antibody (ab92849)

    ab92849 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (neuron)
    Permeabilization Yes - 0.2%Triton
    Specification neuron
    Blocking step Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    Submitted Oct 13 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Monkey Cell (COS7)
    Permeabilization Yes - 0.2%Triton
    Specification COS7
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    Submitted Oct 07 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (brain)
    Loading amount 40 µg
    Specification brain
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

    Abcam user community

    Verified customer

    Submitted Jun 09 2011