Overview

  • Product name
    Anti-ENAH antibody [EPR5660]
    See all ENAH primary antibodies
  • Description
    Rabbit monoclonal [EPR5660] to ENAH
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Human
    Does not react with: Rat
  • Immunogen

    Synthetic peptide corresponding to a sequence within Human ENAH (Q8N8S7).

  • Positive control
    • MDA-MB-435, MCF7, HUVEC and A431 cell lysates; Human Uterus tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa).
IHC-P 1/500 - 1/1000. Antigen rerieval is recommended.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function
      Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes.
    • Tissue specificity
      Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions.
    • Sequence similarities
      Belongs to the Ena/VASP family.
      Contains 1 WH1 domain.
    • Domain
      The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
    • Post-translational
      modifications
      NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cytoplasm. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > filopodium. Cell junction > synapse. Cell junction > focal adhesion. Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses.
    • Information by UniProt
    • Database links
    • Alternative names
      • ENA antibody
      • Enah antibody
      • ENAH_HUMAN antibody
      • MENA antibody
      • NDPP1 antibody
      • Protein enabled homolog antibody
      see all

    Images

    • ab124685, at 1/500 dilution, staining ENAH in formalin-fixed, paraffin-embedded Human uterus tissue, by Immunohistochemistry.
    • All lanes : Anti-ENAH antibody [EPR5660] (ab124685) at 1/1000 dilution

      Lane 1 : MDA-MB-435 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HUVEC cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 67 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Li CF  et al. Characterization of gene amplification-driven SKP2 overexpression in myxofibrosarcoma: potential implications in tumor progression and therapeutics. Clin Cancer Res 18:1598-610 (2012). Read more (PubMed: 22322669) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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