Overview

  • Product name
    Anti-Enah/Vasp-like antibody
    See all Enah/Vasp-like primary antibodies
  • Description
    Goat polyclonal to Enah/Vasp-like
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Orangutan
  • Immunogen

    Synthetic peptide:

    C-SQPPNSSEAGRKP

    , corresponding to internal amino acids 313 - 325 of Human Enah/Vasp-like (NP_057421.1).

  • Positive control
    • Jurkat cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Enah/Vasp-like (EVL) is a member of the Ena/VASP protein family. Ena/VASP proteins are actin-associated proteins involved in a range of processes that require dynamic actin remodeling such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization. There are two different isoforms, generated by alternative splicing.
    • Cellular localization
      Cytoplasm, cytoskeleton. Lamellipodium.
    • Database links
    • Alternative names
      • AI528774 antibody
      • Ena-vasodilator stimulated phosphoprotein antibody
      • Ena/vasodilator stimulated phosphoprotein like antibody
      • Ena/VASP like protein antibody
      • EVL antibody
      • RNB 6 antibody
      • RNB6 antibody
      see all

    Anti-Enah/Vasp-like antibody images

    • Anti-Enah/Vasp-like antibody (ab92280) at 1 µg/ml + Jurkat cell lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 45 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    References for Anti-Enah/Vasp-like antibody (ab92280)

    ab92280 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab92280.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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