Recombinant
RabMAb

Anti-ENC1 antibody [EPR5406(B)] (ab124902)

Overview

  • Product name
    Anti-ENC1 antibody [EPR5406(B)]
    See all ENC1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5406(B)] to ENC1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • Human fetal brain lysate; Human brain tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. May be down-regulated in neuroblastoma tumors. Substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
    • Tissue specificity
      Detected in fetal brain tissue, moderate expression in fetal heart, lung and kidney. Highly expressed in adult brain, particularly high in the hippocampus and amygdala, and spinal chord. Detectable in adult pancreas.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Contains 1 BTB (POZ) domain.
      Contains 6 Kelch repeats.
    • Developmental stage
      Dramatically up-regulated upon neuronal differentiation.
    • Post-translational
      modifications
      Ubiquitinated and probably targeted for proteasome-independent degradation.
    • Cellular localization
      Nucleus matrix. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCL 28 antibody
      • CCL28 antibody
      • Ectoderm neural cortex protein 1 antibody
      • Ectoderm-neural cortex protein 1 antibody
      • Ectodermal neural cortex (with BTB like domain) antibody
      • ENC 1 antibody
      • ENC-1 antibody
      • ENC1 antibody
      • ENC1_HUMAN antibody
      • FLJ39259 antibody
      • Kelch like 35 antibody
      • Kelch like 37 antibody
      • Kelch-like protein 37 antibody
      • KLHL 35 antibody
      • KLHL35 antibody
      • KLHL37 antibody
      • NRPB antibody
      • Nuclear matrix protein NRP/B antibody
      • Nuclear restricted protein BTB domain like (brain) antibody
      • OTTHUMP00000128235 antibody
      • OTTHUMP00000222054 antibody
      • p53 induced gene 10 protein antibody
      • p53-induced gene 10 protein antibody
      • PIG 10 antibody
      • PIG10 antibody
      • TP53I10 antibody
      • Tumor protein p53 inducible protein 10 antibody
      see all

    Anti-ENC1 antibody [EPR5406(B)] images

    • Anti-ENC1 antibody [EPR5406(B)] (ab124902) at 1/1000 dilution + Human brain fetal lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 66 kDa
      Observed band size : 66 kDa
    • ab124902, at 1/100 dilution, staining ENC1 in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-ENC1 antibody [EPR5406(B)] (ab124902)

    ab124902 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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