The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).Can be blocked with Human Endo G peptide (ab9648).
RelevanceEndo G is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
Cellular localizationCytoplasmic, Mitochondrial and Nuclear
All lanes : Anti-Endo G antibody (ab66561) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431) Lane 2 : Spinal Cord (Mouse) Tissue Lysate Lane 3 : Rat Kidney Tissue Lysate Lane 4 : Mouse skeletal muscle tissue lysate - total protein (ab29711) Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 6 : Brain (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution