Overview

  • Product nameAnti-Endo G antibody
    See all Endo G primary antibodies
  • Description
    Rabbit polyclonal to Endo G
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Endo G.

    (Peptide available as ab66560.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Human Heart Tissue Mouse Spinal Cord Tissue Mouse Skeletal Muscle Tissue Rat Brain Tissue HeLa Whole Cell

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab66561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).Can be blocked with Human Endo G peptide (ab9648).

Target

  • RelevanceEndo G is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
  • Cellular localizationMitochondrial
  • Database links
  • Alternative names
    • EndoG antibody
    • Endonuclease G antibody
    • Endonuclease G mitochondrial antibody
    • EndonucleaseG antibody
    • FLJ27463 antibody
    • Mitochondrial endonuclease G antibody
    • NUCG_HUMAN antibody
    see all

Anti-Endo G antibody images

  • All lanes : Anti-Endo G antibody (ab66561) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Spinal Cord (Mouse) Tissue Lysate
    Lane 3 : Rat Kidney Tissue Lysate
    Lane 4 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : Brain (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Endo G antibody (ab66561)

ab66561 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"