Overview

  • Product nameAnti-Endo G antibody
    See all Endo G primary antibodies
  • Description
    Rabbit polyclonal to Endo G
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    GGPRGPGELAKYGLP

    , corresponding to amino acids 55-70 of Human Endo G. (Peptide available as ab9648.)

  • Positive control
    • HepG2 and 3T3 cell lysates
  • General notes


    The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1,2). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndooG also cleaves DNA in vitro (4).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPBS with 0.02% sodium azide
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesThe fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1,2). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndooG also cleaves DNA in vitro (4).
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab9647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 35 kDa.Can be blocked with Human Endo G peptide (ab9648).
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceEndo G is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
  • Cellular localizationMitochondrial
  • Database links
  • Alternative names
    • EndoG antibody
    • Endonuclease G antibody
    • Endonuclease G mitochondrial antibody
    • EndonucleaseG antibody
    • FLJ27463 antibody
    • Mitochondrial endonuclease G antibody
    • NUCG_HUMAN antibody
    see all

Anti-Endo G antibody images

  • Western blot analysis of EndoG in mouse (M) 3T3 and human (H) HepG2 cell lysates with anti-EndoG at 2 µg/ml.
  • ab9647 at 1/50 dilution staining Endo G in human pancreas by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded sections).

  • ICC/IF image of ab9647 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9647, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Endo G antibody (ab9647)

This product has been referenced in:
  • Ovadje P  et al. Evaluation of the efficacy & biochemical mechanism of cell death induction by Piper longum extract selectively in in-vitro and in-vivo models of human cancer cells. PLoS One 9:e113250 (2014). WB . Read more (PubMed: 25401766) »
  • Gouspillou G  et al. Increased sensitivity to mitochondrial permeability transition and myonuclear translocation of endonuclease G in atrophied muscle of physically active older humans. FASEB J 28:1621-33 (2014). IHC ; Human . Read more (PubMed: 24371120) »

See all 11 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12% Gel)
Sample Rat Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Verified customer

Submitted Dec 04 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12% Gel)
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 04 2014

Merci de votre intérêt pour ab9647. Comme discuté au téléphone, cet anticorps n’a pas encore été testé en cytométrie de flux . Cependant, si vous souhaitez tester ab9647 en cytométrie de flux, il me sera possible de vous offrir une remise. Après soumis...

Read More

Please refer to the "Protocols" section (IHC, Western blot, ELISA, FACS etc) on the right hand side panel of the Abcam homepage. You may find some useful information there.

All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). According to the datasheet, this antibody has not been tested for IP application.

We have never tested this antibody in IHC, therefore we can't guarantee that it will work. To the best of our knowledge, no-one else has tested it in IHC, either.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"