Overview

  • Product nameAnti-Endo G antibody [EP1665Y]
    See all Endo G primary antibodies
  • Description
    Rabbit monoclonal [EP1665Y] to Endo G
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues on the C terminus of human Endo G.

  • Positive control
    • HepG2 cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • RelevanceEndo G is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
    • Cellular localizationMitochondrial
    • Database links
    • Alternative names
      • EndoG antibody
      • Endonuclease G antibody
      • Endonuclease G mitochondrial antibody
      • EndonucleaseG antibody
      • FLJ27463 antibody
      • Mitochondrial endonuclease G antibody
      • NUCG_HUMAN antibody
      see all

    Anti-Endo G antibody [EP1665Y] images



    • Predicted band size : 36 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Endo G HAP1 cell lysate (20 µg)
      Lane 3: Mouse liver tissue lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab76122 observed at 29 kDa. Red - loading control, ab7291, observed at 52 kDa.

      ab76122 was shown to specifically react with Endo G when Endo G knockout samples were used. Wild-type and Endo G knockout samples were subjected to SDS-PAGE. ab76122 and ab7291 (loading control to alpha tubulin) were diluted 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Anti-Endo G antibody [EP1665Y] (ab76122) at 1/2000 dilution + HepG2 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/1000 dilution
      Developed using the ECL technique

      Predicted band size : 36 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    References for Anti-Endo G antibody [EP1665Y] (ab76122)

    This product has been referenced in:
    • Pardo R  et al. EndoG Knockout Mice Show Increased Brown Adipocyte Recruitment in White Adipose Tissue and Improved Glucose Homeostasis. Endocrinology 157:3873-3887 (2016). Mouse . Read more (PubMed: 27547848) »

    See 1 Publication for this product

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