Endogenous Avidin/Biotin Blocking Kit (ab64212)

Overview

  • Product name
    Endogenous Avidin/Biotin Blocking Kit
    See all Endogenous Avidin+Biotin Blocking System kits
  • Product overview

    In the demonstration of tissue antigens by Immunohistochemistry, the evaluation of specific staining is sometimes impeded by the presence of endogenous biotin, which may lead to an erroneous interpretation or false positive results. This blocking system is intended for use in immunohistochemical staining to block nonspecific staining due to endogenous biotin during the IHC detection of antigens.

  • Tested applications
    Suitable for: IHC-P, ICC/IF, IHC-Frmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Storage buffer
    Preservative: 0.08% Sodium azide
    Constituent: Avidin
  • Components 15 ml 15 ml
    Avidin Block 1 x 15ml 1 x 15ml
    Biotin Block 1 x 15ml 1 x 15ml
  • Research areas
  • Relevance
    Some cells, and tissues such as kidney, liver and spleen, contain endogenous biotin. Using an avidin-biotin staining method may result in high, non-specific background staining. A significant reduction of this non-specific background can be obtained by pre-treatment of cells/tissues with avidin/biotin blocking reagents prior to the incubation of biotinylated antibody.

Applications

Our Abpromise guarantee covers the use of ab64212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Images

  • Immunohistochemical analysis staining HTRA1 in mouse bone tissue sections. Tissue sections were dewaxed, rehydrated and treated with Endogenous Avidin/Biotin Blocking Kit (ab64212), 3% H2Oand normal swine serum. Tissue sections were then incubated with a polyclonal anti-HTRA1 antibody for 1 hour at 37°C. After PBS wash, tissue sections are incubated with biotinylated swine anti-rabbit IgG for 45 minutes at 37°C and further incubated for 30 minutes after washing. With Vectastatin. Sections were developed using DAB and counterstained using Harris modified hematoxylin. 

References

This product has been referenced in:
  • Filliat G  et al. Role of HTRA1 in bone formation and regeneration: In vitro and in vivo evaluation. PLoS One 12:e0181600 (2017). IHC . Read more (PubMed: 28732055) »
  • Planque SA  et al. Physiological IgM class catalytic antibodies selective for transthyretin amyloid. J Biol Chem 289:13243-58 (2014). Read more (PubMed: 24648510) »

See all 2 Publications for this product

Customer reviews and Q&As

helpful kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
We used this kit to block the non-specific binding of avidin or biotinylated reagents with the endogenous biotin, also their receptors and the avidin binding sites in the tissue.
Its works good and is easy to use.
We used the kit as a ready to use-kit and incubated the samples first with the avidin block for 10minutes and after a washing step with the bioin block for 10minutes.
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Abcam user community

Verified customer

Submitted Mar 12 2018


I can confirm that the Avidin Block and Biotin block provided with this kit come ready to use. They should not be diluted.

Once the sections are cut into sections and placed on a slide, the avidin and biotin blocking is done as part of th...

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Thank you for contacting us.

I would not recommend using the normal serum after biotin blocking for fear of introducing biotin to the samples. However if you would like, you may block with normal serum (5-10%) prior to biotin/avidin blocki...

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Thank you for your reply.

In this case I would recommend that you quench endogenous biotin/avidin in your tissue prior to staining. We recommend use of our Endogenous Avidin/Biotin Blocking Kit (ab64212).

After blocking with th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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