Overview

  • Product nameEndogenous Avidin/Biotin Blocking Kit
    See all Endogenous Avidin+Biotin Blocking System kits
  • Product overview

    In the demonstration of tissue antigens by Immunohistochemistry, the evaluation of specific staining is sometimes impeded by the presence of endogenous biotin, which may lead to an erroneous interpretation or false positive results. This blocking system is intended for use in immunohistochemical staining to block nonspecific staining due to endogenous biotin during the IHC detection of antigens.

  • Notes

    In the demonstration of tissue antigens by Immunohistochemistry, the evaluation of specific staining is sometimes impeded by the presence of endogenous biotin, which may lead to an erroneous interpretation or false positive results. This blocking system is intended for use in immunohistochemical staining to block nonspecific staining due to endogenous biotin during the IHC detection of antigens.

  • Tested applicationsSuitable for: IHC-Pmore details

Properties

  • RelevanceSome cells, and tissues such as kidney, liver and spleen, contain endogenous biotin. Using an avidin-biotin staining method may result in high, non-specific background staining. A significant reduction of this non-specific background can be obtained by pre-treatment of cells/tissues with avidin/biotin blocking reagents prior to the incubation of biotinylated antibody.

Applications

Our Abpromise guarantee covers the use of ab64212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

References for Endogenous Avidin/Biotin Blocking Kit (ab64212)

This product has been referenced in:
  • Planque SA  et al. Physiological IgM class catalytic antibodies selective for transthyretin amyloid. J Biol Chem 289:13243-58 (2014). Read more (PubMed: 24648510) »

See 1 Publication for this product

Product Wall


I can confirm that the Avidin Block and Biotin block provided with this kit come ready to use. They should not be diluted.

Once the sections are cut into sections and placed on a slide, the avidin and biotin blocking is done as part of th...

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I would not recommend using the normal serum after biotin blocking for fear of introducing biotin to the samples. However if you would like, you may block with normal serum (5-10%) prior to biotin/avidin blocki...

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Thank you for your reply.

In this case I would recommend that you quench endogenous biotin/avidin in your tissue prior to staining. We recommend use of our Endogenous Avidin/Biotin Blocking Kit (ab64212).

After blocking with th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"