The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionSelectively cleaves DNA at the second phosphodiester bond 3' to hypoxanthine- and uracil-containing nucleotides. Shows higher activity towards single-stranded than double-stranded DNA and towards hypoxanthine than uracil.
Tissue specificityHighest levels detected in liver with high levels also found in heart, kidney and testis. Expressed at low levels in brain.
Sequence similaritiesBelongs to the endonuclease V family.