Clone 1837-46 is directed against the endostatin portion (the carboxy-terminal domain) of collagen XVIII. Endostatin is a powerful inhibitor of endothelial cell proliferation and angiogenesis and shows widespread distribution, mainly in vessel walls and some other basement membrane zones.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Purification notesPurified from tissue culture supernatant.
Primary antibody notesClone 1837-46 is directed against the endostatin portion (the carboxy-terminal domain) of collagen XVIII. Endostatin is a powerful inhibitor of endothelial cell proliferation and angiogenesis and shows widespread distribution, mainly in vessel walls and some other basement membrane zones.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml.
Fixation: acetone 10 minutes.
Use a concentration of 10 µg/ml.
Microwave irradiation (2 x 7min) in citrate buffer is recommended for antigen retrieval.
FunctionCOLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.
Tissue specificityPresent in multiple organs with highest levels in liver, lung and kidney.
Involvement in diseaseDefects in COL18A1 are a cause of Knobloch syndrome (KNO) [MIM:267750]. KNO is an autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele.
Sequence similaritiesBelongs to the multiplexin collagen family. Contains 1 FZ (frizzled) domain. Contains 1 TSP N-terminal (TSPN) domain.
Post-translational modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Cellular localizationSecreted > extracellular space > extracellular matrix.
IHC image of ab15685 staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15685, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Endostatin antibody [1837-46] (ab15685)
has not yet been referenced specifically in any publications.
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