Overview

  • Product nameAnti-Endostatin antibody [4i37]
    See all Endostatin primary antibodies
  • Description
    Mouse monoclonal [4i37] to Endostatin
  • Tested applicationsSuitable for: WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Full-length recombinant murine endostatin

  • Positive control
    • 3T3 cell lysates with 50ng added recombinant human endostatin.

Properties

Applications

Our Abpromise guarantee covers the use of ab64569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). An unidentifed protein is also weakly detected at 54kDa.
IHC-Fr Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.

Target

  • FunctionCOLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.
    Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.
  • Tissue specificityPresent in multiple organs with highest levels in liver, lung and kidney.
  • Involvement in diseaseDefects in COL18A1 are a cause of Knobloch syndrome (KNO) [MIM:267750]. KNO is an autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele.
  • Sequence similaritiesBelongs to the multiplexin collagen family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 TSP N-terminal (TSPN) domain.
  • Post-translational
    modifications
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 collagen type 18 (XVIII)(COL18A1) antibody
    • Alpha 1 type XVIII collagen antibody
    • Antiangiogenic agent antibody
    • COIA1_HUMAN antibody
    • COL15A1 antibody
    • Col18a1 antibody
    • Collagen alpha 1(XV) chain antibody
    • Collagen alpha 1(XVIII) chain antibody
    • Collagen alpha-1(XV) chain antibody
    • Collagen type XV proteoglycan antibody
    • Collagen type XVIII alpha 1 antibody
    • Collagen XV, alpha 1 polypeptide antibody
    • Collagen, type XV, alpha 1 antibody
    • Endostatin antibody
    • Endostatin XV antibody
    • FLJ27325 antibody
    • FLJ34914 antibody
    • FLJ38566 antibody
    • KNO antibody
    • KNO1 antibody
    • KS antibody
    • MGC74745 antibody
    • Multi functional protein MFP antibody
    • OTTHUMP00000021782 antibody
    • OTTHUMP00000115472 antibody
    • OTTHUMP00000115473 antibody
    see all

References for Anti-Endostatin antibody [4i37] (ab64569)

This product has been referenced in:
  • Garcia PV  et al. Increased toll-like receptors and p53 levels regulate apoptosis and angiogenesis in non-muscle invasive bladder cancer: mechanism of action of P-MAPA biological response modifier. BMC Cancer 16:422 (2016). WB . Read more (PubMed: 27389279) »
  • Ma S  et al. D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth. Oncotarget 6:8606-20 (2015). Read more (PubMed: 25825982) »

See all 7 Publications for this product

Product Wall

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This antibody has not been tested for crossreactivity against other CDAFs, such as restin, arrestin, canstatin, or tumstatin.

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In testing, I can confirm that ab64569 Anti-Endostatin antibody [4i37]has been used successfully in IHCon human colon tissue (endothethial cells). These should be a good positive control. The negative control usedwa...

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I am pleased to provide the following information and protocol for you for ab64569.

I can confirm this antibody has been tested in mouse kidney and heart, and human kidney. I am sorry it has regrettably...

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I am very pleased to hear you would like to accept our offer and test ab64569 in rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"