The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant mouse endostatin is approximately 6 ng/well.
WB: Use at a concentration of 1 - 2 µg/ml. The detection limit for recombinant mouse endostatin is approximately 50 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. Predicted molecular weight: 22 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCOLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.
Tissue specificityPresent in multiple organs with highest levels in liver, lung and kidney.
Involvement in diseaseDefects in COL18A1 are a cause of Knobloch syndrome (KNO) [MIM:267750]. KNO is an autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele.
Sequence similaritiesBelongs to the multiplexin collagen family. Contains 1 FZ (frizzled) domain. Contains 1 TSP N-terminal (TSPN) domain.
Post-translational modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Cellular localizationSecreted > extracellular space > extracellular matrix.