Rat monoclonal [1F10] to Endothelial Cell (Biotin)
Specificityab51675 recognizes an antigen expressed on human continuous blood endothelial cells which are fully differentiated. The antibody staining is restricted to endothelial cells and does not stain any other cell in tissues or in peripheral blood. On sinusoidal endothelial cells of the liver or lymphatic organs the antigen is inconsistently expressed or absent.
On isolated cells, the antigen is found in variable numbers on freshly isolated umbilical vein endothelial cells (HUVECs). Antigen expression on cultured HUVECs is dependent on the culture conditions. Best results were obtained (40-70% pos.) by daily renewal of FCS containing medium supplemented with endothelial cell conditioned supernatant whereas commercially available endothelial cell media apparently have no influence
on the antigen expression.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Application notesIs unsuitable for IHC-P.
RelevanceThe endothelium is a single layer of cells lining various organs and cavities of the body, especially the blood vessels, heart, and lymphatic vessels.Endothelial cells line the entire circulatory system, from the heart to the smallest capillary.
Endothelial cells are involved in many aspects of vascular biology, such as vasoconstriction and vasodilation, and hence the control of blood pressure; blood clotting (thrombosis & fibrinolysis); atherosclerosis; formation of new blood vessels (angiogenesis) and inflammation and swelling (oedema). Endothelial cells also control the passage of materials, and the transit of white blood cells, into and out of the bloodstream.
References for Anti-Endothelial Cell antibody [1F10] (Biotin) (ab51675)
This product has been referenced in:
Goerdt S et al. Characterization and differential expression of an endothelial cell-specific surface antigen in continuous and sinusoidal endothelial, in skin vascular lesions and in vitro. Exp Cell Biol57:185-92 (1989).
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