Anti-Endothelial Cell antibody [RECA-1] (ab9774)

Overview

  • Product nameAnti-Endothelial Cell antibody [RECA-1]
    See all Endothelial Cell primary antibodies
  • Description
    Mouse monoclonal [RECA-1] to Endothelial Cell
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-G, IHC-Fr, IHC-FrFlmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Peripheral and mesenteric lymph nodes from AO rat

  • Positive control
    • Rat brain FFPE section (IHC)
  • General notes

    This antibody is available as an azide-free product (see ab172579).

    RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognises a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Please note that some batches of ab9774 may contain 0.4M arginine. Please contact Scientific Support for further information.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesRECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognises a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.
  • ClonalityMonoclonal
  • Clone numberRECA-1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. Fix tissue in 2% paraformaldehyde in PBS (pH 7.4) for 3 hours and maintain overnight in 6% sucrose in PBS, rinse with acetone, and embed in Technovit 8100 (glycol methacrylate). Do not perform antigen retrieval (see Duijvestejn AM et al. for details).
IHC-P Use a concentration of 10 µg/ml.
IHC-G 1/10 - 1/50.
IHC-Fr 1/50.
IHC-FrFl Use at an assay dependent concentration.

Target

  • RelevanceThe endothelium is the layer of thin specialized epithelium, comprising a simple squamous layer of cells that line the interior surface of blood vessels, forming an interface between circulating blood in the lumen and the rest of the vessel wall (simple squamous epithelium). Endothelial cells line the entire circulatory system, from the heart to the smallest capillary. Endothelial cells are involved in many aspects of vascular biology, such as vasoconstriction and vasodilation, and hence the control of blood pressure; blood clotting (thrombosis & fibrinolysis); atherosclerosis; formation of new blood vessels (angiogenesis) and inflammation and swelling (oedema). Endothelial cells also control the passage of materials, and the transit of white blood cells, into and out of the bloodstream.

Anti-Endothelial Cell antibody [RECA-1] images

  • IHC image of Endothelial Cell staining in Rat brain FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9774, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • ab9774 staining cells of rat liver sections by IHC-Fr.  Cells were acetone fixed and blocked with 1% BSA for 5 minutes at 25°C prior to incubating with ab9774 diluted 1/25 for 30 minutes at 25°C.  An Alexa Fluor® 488 conjugated goat anti-mouse antibody was used as the secondary.

    Double IIF labeling with RECA-1 (Green) and dipeptidyl peptidase IV (Red)

    See Abreview

  • ab9774 staining endothelial cells in Rat lung tissue by Immunohistochemistry (Frozen sections). The sections were fixed in acetone prior to blocking with 100% SuperBlock Blocking Buffer for 20 minutess at 23°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-Mouse polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

References for Anti-Endothelial Cell antibody [RECA-1] (ab9774)

This product has been referenced in:
  • Kimura-Ohba S  et al. Transient increase of fractional anisotropy in reversible vasogenic edema. J Cereb Blood Flow Metab 36:1731-1743 (2016). Read more (PubMed: 26865662) »
  • Fukuda Y  et al. Intra-arterial transplantation of low-dose stem cells provides functional recovery without adverse effects after stroke. Cell Mol Neurobiol 35:399-406 (2015). Read more (PubMed: 25398358) »

See all 19 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Liver)
Permeabilization No
Specification Liver
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 20°C
Fixative Acetone
Username

Dr. Fabio Marongiu

Verified customer

Submitted Oct 20 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain tissue)
Antigen retrieval step None
Specification Brain tissue
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10 · Temperature: 24°C
Fixative Formaldehyde
Username

Sumit Sarkar

Verified customer

Submitted Aug 14 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate pH=6
Sample Rat Tissue sections (omentum)
Specification omentum
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 09 2014

Unfortunately this clone, RECA-1, for anti-Anti-Endothelial Cell antibody is produced only in mouse. I have reviewed other products on our catalog and it appears that none of our other Anti-Endothelial Cell antibodies are produced in rabbit either. I a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Heart)
Specification Heart
Fixative 10% Neutral Buffered Formalin, 24h
Antigen retrieval step Enzymatic
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Oct 23 2012

Thank you for contacting us.

ab9774 is a well characterized antibody is fully guaranteed to detect the endothelial cells. Please use the product without any hesitation.

ab22492 is also reactive against vascular endothelium so this pro...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Kidneys)
Specification Kidneys
Fixative Formaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Kidneys)
Specification Kidneys
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 30 2012

Thank you for contacting us.
The clone number for ab9774 is RECA-1.
http://www.abcam.com/endothelial-cell-antibody-reca-1-ab9774.html
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more ad...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"