Anti-Endothelin 1 antibody (ab117757)

Overview

  • Product nameAnti-Endothelin 1 antibody
    See all Endothelin 1 primary antibodies
  • Description
    Rabbit polyclonal to Endothelin 1
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to CS-Abu-SSLMDKE-Abu-VYF-Abu-HLDIIW of Mouse Endothelin 1.

  • Positive control
    • Mouse dorsal root ganglia tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab117757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 23 kDa.
IHC-P 1/2000.
ICC 1/5000.

Target

Anti-Endothelin 1 antibody images

  • ab117757 at 1/2000 dilution staining Endothelin 1 in Mouse Dorsal root ganglia by Immunohistochemistry.

References for Anti-Endothelin 1 antibody (ab117757)

ab117757 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and your interest in our products.

I am sorry for the delay in getting back to you. I can confirm that the Anti-Endothelin 1 antibody (ab117757) has been used to perform IHC staining with paraffin embedded tissue wi...

Read More

Wenn wir keine genaueren Angaben zum Protokoll machen (wie in diesem Fall), bedeutet dies, dass Sie eigentlich ohne Probleme Ihr Standard-Protokoll verwenden können (Optimierungsschritte eingeschlossen).


Unfortunately, we do not have the blocking peptide for ab117757 available for sale. I apologize for saying it would be likely that we can make it available. If you cannot find an alternative blocking peptide source that overlaps the immunogen sequ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (liver)
Loading amount 30 µg
Specification liver
Treatment 2mg/kg LPS 6h
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 20 2012

Thank you for your inquiry and I am sorry to hear that you are having trouble with this antibody. I've copied our protocol below. Please letme know if this is similar to what you've tried.


Western Blot Protocol


Polyacryl...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"