Anti-Endothelin 1 antibody (ab117757)
Key features and details
- Rabbit polyclonal to Endothelin 1
- Suitable for: IHC-P, WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Endothelin 1 antibody
See all Endothelin 1 primary antibodies -
Description
Rabbit polyclonal to Endothelin 1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide conjugated to KLH, corresponding to CS-Abu-SSLMDKE-Abu-VYF-Abu-HLDIIW of Mouse Endothelin 1.
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Positive control
- WB: HEK-293, HUVEC, HAEC and SK-BR-3 cell lysates IHC-P: Mouse Dorsal root ganglia ICC: A549 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/2000.
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WB | (1) |
1/3000 - 1/5000. Predicted molecular weight: 24 kDa.
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ICC |
1/250.
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Notes |
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IHC-P
1/2000. |
WB
1/3000 - 1/5000. Predicted molecular weight: 24 kDa. |
ICC
1/250. |
Target
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Function
Endothelins are endothelium-derived vasoconstrictor peptides. -
Tissue specificity
Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. -
Sequence similarities
Belongs to the endothelin/sarafotoxin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1906 Human
- Entrez Gene: 13614 Mouse
- Omim: 131240 Human
- SwissProt: P05305 Human
- SwissProt: P22387 Mouse
- Unigene: 511899 Human
- Unigene: 713645 Human
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Alternative names
- ARCND3 antibody
- Big endothelin-1 antibody
- EDN1 antibody
see all
Images
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All lanes : Anti-Endothelin 1 antibody (ab117757) at 1/3000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : HAEC cell lysate
Lane 3 : SK-BR-3 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Recombinant Secondary Antibody, HRP at 1/4000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa -
ab117757 at 1/2000 dilution staining Endothelin 1 in Mouse Dorsal root ganglia by Immunohistochemistry.
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Immunocytochemistry analysis of 70% confluent log phase A549 cells labeling Endothelin 1 with ab117757. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab117757 at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin at 1/300. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab117757 has been referenced in 26 publications.
- Weigert A et al. Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system. Nat Commun 13:6078 (2022). PubMed: 36241617
- Sutovska H et al. Artificial light at night suppresses the expression of sarco/endoplasmic reticulum Ca2+ -ATPase in the left ventricle of the heart in normotensive and hypertensive rats. Exp Physiol 106:1762-1771 (2021). PubMed: 34089548
- Neder TH et al. Endothelin receptors in renal interstitial cells do not contribute to the development of fibrosis during experimental kidney disease. Pflugers Arch 473:1667-1683 (2021). PubMed: 34355294
- Karoor V et al. Evidence supporting a role for circulating macrophages in the regression of vascular remodeling following sub-chronic exposure to hemoglobin plus hypoxia. Pulm Circ 11:20458940211056806 (2021). PubMed: 34777787
- Liu Z et al. USP22 regulates the formation and function of placental vasculature during the development of fetal growth restriction. Placenta 111:19-25 (2021). PubMed: 34130183