Overview

  • Product name
    Anti-Endothelin 1 antibody
    See all Endothelin 1 primary antibodies
  • Description
    Rabbit polyclonal to Endothelin 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Endothelin 1 aa 111-138 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P05305

  • Positive control
    • A549 cell line lysate, Human placenta tissue, A549 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab170215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 24 kDa.
ICC/IF 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Endothelins are endothelium-derived vasoconstrictor peptides.
  • Tissue specificity
    Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
  • Sequence similarities
    Belongs to the endothelin/sarafotoxin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARCND3 antibody
    • Big endothelin-1 antibody
    • EDN1 antibody
    • EDN1_HUMAN antibody
    • ET-1 antibody
    • ET1 antibody
    • HDLCQ7 antibody
    • PPET1 antibody
    • Preproendothelin 1 antibody
    • Preproendothelin-1 antibody
    • QME antibody
    see all

Images

  • Flow Cytometrical analysis of permeabilized A549 cells with ab170215 antibody at a dilution of 1/10 (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunofluorescent analysis of A549 cells labeling Endothelin 1 with ab170215 at 1/10 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI staining.

  • Immunofluorescent analysis of A549 cells labeling Endothelin 1 with ab170215 at 1/10 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and  DAPI staining.

  • Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling Endothelin 1 with ab170215 at 1/50.

  • Anti-Endothelin 1 antibody (ab170215) at 1/100 dilution + A549 cell line lysate at 35 µg

    Predicted band size: 24 kDa

References

ab170215 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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