Anti-Endothelin A Receptor antibody (ab117521)

Overview

  • Product nameAnti-Endothelin A Receptor antibody
    See all Endothelin A Receptor primary antibodies
  • Description
    Rabbit polyclonal to Endothelin A Receptor
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    KNQEQNNHNTERSSHKDSMN

    conjugated to KLH, corresponding to C terminal amino acids 408-427 of Mouse Endothelin A Receptor.

  • Positive control
    • Mouse dorsal root ganglia; Mouse heart tissue; Endothelin A Receptor transfected cells; Mouse heart lysate
  • General notes

    We have received mixed feedback about mouse cross reactivity, some lot works and some doesn't so we no longer guarantee this species.

Properties

Applications

Our Abpromise guarantee covers the use of ab117521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 49 kDa.
IHC-P 1/2000.
ICC/IF 1/5000.

Target

  • FunctionReceptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3.
  • Tissue specificityIsoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ednra antibody
    • EDNRA_HUMAN antibody
    • Endothelin 1 receptor precursor antibody
    • Endothelin 1 specific receptor antibody
    • Endothelin A receptor antibody
    • Endothelin ET A receptor antibody
    • Endothelin receptor ET1 specific type antibody
    • Endothelin receptor subtype A antibody
    • Endothelin receptor type A antibody
    • Endothelin-1 receptor antibody
    • ET A antibody
    • ET-A antibody
    • ETA antibody
    • ETA R antibody
    • ETA-R antibody
    • ETAR antibody
    • ETRA antibody
    • G protein coupled receptor antibody
    • hET AR antibody
    • hET-AR antibody
    • MFDA antibody
    see all

Anti-Endothelin A Receptor antibody images

  • ab117521, at 1/5000 dilution, staining Endothelin A Receptor in HEK293 transfected with Endothelin A receptor cells  by Immunocytochemistry.

  • ab117251, at 1/2000 dilution, staining Endothelin A Receptor in the atrium of mouse heart by Immunohistochemistry.
  • Anti-Endothelin A Receptor antibody (ab117521) at 1/5000 dilution + Mouse heart lysate

    Predicted band size : 49 kDa

References for Anti-Endothelin A Receptor antibody (ab117521)

ab117521 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Cryopreserved embryonic cerebellar neurons (QBMcel)
Permeabilization No
Specification Cryopreserved embryonic cerebellar neurons (QBMcel
Fixative paraformaldehyde with picric acid
Username

Ms. Babben Tinner

Verified customer

Submitted Sep 29 2015

Thank you very much for your call today and for letting us know about the trouble with the staining results.

Please keep me updated about the results after trying the protocol alterations that we discussed. I look forward to hearing from you,...

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DISCOUNT CODE: XXXX
Expiration date: 2012-10-02

Thank you for calling yesterday. I am very pleased to hear that you would like to acceptmy offer and testthe anti-Endothelin A Receptor antibody (ab117521)with frozen tissue. This code abo...

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Thank you for your follow up email and I am sorry that I did not notice it until I had already sent your reply.

You are correct in thinking that the anti-Endothelin A Receptor antibody (ab85163) has been used successfully in staining frozen t...

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Thank you for your call today and for letting us know about the trouble with ab117521.

As we discussed, I would recommend permeabilizing the cells with 0.1-0.2% Tween 20 instead of with Triton, and using a 1:500-1:1000 dilution of antibody. P...

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Thank you for contacting Abcam regarding our anti-Endothelin Receptor A antibodies. Regarding these antibodies, all of the information that is currently available can be found on the product datasheets on our website. I have summarized the answers to y...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"