Overview

  • Product nameAnti-Endothelium antibody [EN4]
    See all Endothelium primary antibodies
  • Description
    Mouse monoclonal [EN4] to Endothelium
  • SpecificityThis antibody stains all human blood vessels including brain microvessels. Both large and small vessels are equally reactive. The EN4 antigen is preserved in endothelial cells in culture unlike the PAL-E antigen (ab8086) which is lost. It stains strongly murine fibroblasts transfected with the human CD31 gene. On surface-iodinated Jurkat T cells it recognizes the 130 kD CD31 antigen. EN4 also binds to guinea-pig and cat endothelium, but not to rabbit, bovine, sheep, dog, rat or mice endothelial cells. No other structures in the skin, heart, kidney, tonsils or spleen are stained with this antibody.
  • Tested applicationsSuitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Guinea pig, Cat, Human
    Does not react with: Mouse, Sheep, Rabbit, Cow, Dog
  • General notes


    The antibody is useful for immunohistological staining of endothelia. Lymphatic endothelium is stained (unlike the PAL-E antibody). Kaposi's sarcoma and other vascular tumors of endothelial cell origin are also stained.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: 0.1% BSA
  • PurityTissue culture supernatant
  • Primary antibody notesThe antibody is useful for immunohistological staining of endothelia. Lymphatic endothelium is stained (unlike the PAL-E antibody). Kaposi's sarcoma and other vascular tumors of endothelial cell origin are also stained.
  • ClonalityMonoclonal
  • Clone numberEN4
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20.
WB Use at an assay dependent concentration. PubMed: 21645391
IHC-Fr 1/20.

Target

  • RelevanceThe endothelium is located at the interface between the blood and the vessel wall. The cells are in close contact and form a slick layer that prevents blood cell interaction with the vessel wall as blood moves through the vessel lumen. The endothelium consists of simple squamous epithelium that lines the lumen of all blood vessels. It plays a critical role in the mechanics of blood flow, the regulation of coagulation, leukocyte adhesion, and vascular smooth muscle cell growth, and also serves as a barrier to the transvascular diffusion of liquids and solutes. For years the endothelium was thought of as an inert single layer of cells that passively allow the passage of water and other small molecules across the vessel wall. However, this dynamic tissue performs many other active functions, such as the secretion and modification of vasoactive substances and the contraction and relaxation of vascular smooth muscle.

Anti-Endothelium antibody [EN4] images

  • ab8087 staining Endothelium in Human lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and methanol, and blocked with 10% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50) overnight at 4°C. A TRITC-conjugated Goat anti-mouse IgG (1/50) was used as the secondary antibody.

References for Anti-Endothelium antibody [EN4] (ab8087)

This product has been referenced in:
  • Lasagna-Reeves CA  et al. Tau oligomers impair memory and induce synaptic and mitochondrial dysfunction in wild-type mice. Mol Neurodegener 6:39 (2011). WB ; Mouse . Read more (PubMed: 21645391) »
  • Péqueux C  et al. Oxytocin receptor pattern of expression in primary lung cancer and in normal human lung. Lung Cancer 50:177-88 (2005). IHC-Fr ; Human . Read more (PubMed: 16043261) »

See all 5 Publications for this product

Product Wall

Thank you for your enquiry. The immunogen for ab8087 is endothelium. I hope that this information helps. Please do not hesitate to contact us for advice in the future.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"