• Product nameAnti-Endothelium antibody [PAL-E]
    See all Endothelium primary antibodies
  • Description
    Mouse monoclonal [PAL-E] to Endothelium
  • SpecificityReactivity with: Lymphatic endothelium :not detectable. Sinus histiocytes :not detectable. Megakaryocytes :not detectable.
  • Tested applicationsSuitable for: IHC-Frmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Sheep, Rabbit, Goat, Dog, Human, Pig
    Does not react with: Mouse, Rat, Chicken
  • Immunogen

    Human endothelium

  • General notes

    The antibody only stains human and various animal (goat, rabbit, pig, dog) blood vessels with the exception of arteries.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • PurityTissue culture supernatant
  • Primary antibody notesThe antibody only stains human and various animal (goat, rabbit, pig, dog) blood vessels with the exception of arteries.
  • ClonalityMonoclonal
  • Clone numberPAL-E
  • IsotypeIgG2a
  • Research areas


Our Abpromise guarantee covers the use of ab8086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100. Fix with acetone.
  • Application notesIs unsuitable for WB.
  • Target

    • RelevanceThe endothelium is located at the interface between the blood and the vessel wall. The cells are in close contact and form a slick layer that prevents blood cell interaction with the vessel wall as blood moves through the vessel lumen. The endothelium consists of simple squamous epithelium that lines the lumen of all blood vessels. It plays a critical role in the mechanics of blood flow, the regulation of coagulation, leukocyte adhesion, and vascular smooth muscle cell growth, and also serves as a barrier to the transvascular diffusion of liquids and solutes. For years the endothelium was thought of as an inert single layer of cells that passively allow the passage of water and other small molecules across the vessel wall. However, this dynamic tissue performs many other active functions, such as the secretion and modification of vasoactive substances and the contraction and relaxation of vascular smooth muscle.

    References for Anti-Endothelium antibody [PAL-E] (ab8086)

    This product has been referenced in:

    See all 5 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (endothelial (HUVECs))
    Specification endothelial (HUVECs)
    Fixative Paraformaldehyde
    Permeabilization Yes - triton-x100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C

    Dr. S Della Bella

    Verified customer

    Submitted Nov 26 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Sheep Tissue sections (Brain)
    Specification Brain
    Fixative Acetone
    Permeabilization Yes - triton-x
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 15°C

    Abcam user community

    Verified customer

    Submitted Aug 24 2012

    I am very pleased to hear you would like to take part in the testing discount scheme. I have generated the discount code as follows:
    Discount code: xxxxxxxxx Expiration date: 2nd December 2012
    Please quote this code in the "Additional comment...

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    Thank you for contacting us and your interest in our products.
    As you have seen from the datasheet of the anti-Endothelium antibody [PAL-E] (ab8086), the antibody has not as yet been used in detecting the ovine protein or IHC of paraffin embedded ...

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    DISCOUNT CODE: 0000000

    Expiration date: 03. November 2012
    I am very pleased to hear your customer would like to accept our offer and test ab8086 in ICC/IF.
    This code will give your customer 1 free primary antibody before the expirat...

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    Thank you and your customer very much for your interest in ab8086.
    To our knowledge, ab8086 has not been tested in ICC/IF. Therefore, I can offer a discount off a future purchase if your customer buys ab8086 now, tests it in ICC/IF and submits fee...

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    Thank you for your email.
    I can confirm that you are correct and ab8086 is the same clone as ab128058 and therefore the best alternative for your customer.
    Please let me know what application and species your customer is using if you have any...

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    Thank you for your enquiry. All the information we having regarding this antibody is located on the online datasheet. I am sorry but we do not have a protocol for tagging ICG with PAL-E. If you have any more questions, please contact us again.

    Dear Kavita datasheets. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you dec...

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    We do not have any data on the antibodies use in formalin-fixed paraffin embedded tissue and believe that it has not yet been tested in this application. If we can find further information we will contact you with this.