Anti-Engrailed and Invected antibody [4D9] (ab12454)

Overview

  • Product name
    Anti-Engrailed and Invected antibody [4D9]
  • Description
    Mouse monoclonal [4D9] to Engrailed and Invected
  • Specificity
    This antibody recognizes both the engrailed and invected gene products of Drosophila. The antibody is useful for examining the patterning of segmentation from the cellular blastoderm stage onward. Staining is also seen in a large number of CNS neurons including most of the median neuroblast progeny as well as a small number of PNS neurons.
  • Tested applications
    Suitable for: IHC-FoFrmore details
  • Species reactivity
    Reacts with: Chicken, Xenopus laevis, Drosophila melanogaster, Zebrafish, Crayfish
    Does not react with: Mouse
  • Immunogen

    C-terminal two-thirds of the invected protein were generated in E. coli with the T7 polymerase expression system.

  • Epitope
    Epitope has been localized to residues 38-58 of the homeodomain.
  • General notes

    This antibody clone is manufactured by Abcam.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    4D9
  • Myeloma
    NS1
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/200. Fix with 4% paraformaldehyde in PBS for 1 hour.

Target

  • Relevance
    In Drosophila, the Engrailed gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Expression studies have suggested that Engrailed may also function early in organizing the preblastoderm and later in neurogenesis. Engrailed encodes a homeodomain-containing protein that can function as a transcription factor. Invected is homologous to Engrailed and the gene lies adjacent to that for Engrailed. The function of Invected is unclear.
  • Database links
    • Alternative names
      • En antibody
      • Eng antibody
      • Engrailed antibody
      • Engrailed/Invected antibody
      • Homeobox protein invected antibody
      • Inv antibody
      • Invected antibody
      see all

    Anti-Engrailed and Invected antibody [4D9] images

    • ab12454 used in immunohistochemistry at 1/200 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.

      Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining.Visualisation was with DAB.

    • ab12454 used in immunofluorescence at 1/200 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.

      Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining.

    References for Anti-Engrailed and Invected antibody [4D9] (ab12454)

    This product has been referenced in:
    • Baratte S  et al. Engrailed in cephalopods: a key gene related to the emergence of morphological novelties. Dev Genes Evol 217:353-62 (2007). Read more (PubMed: 17394016) »
    • Harzsch S Evolution of identified arthropod neurons: the serotonergic system in relation to engrailed-expressing cells in the embryonic ventral nerve cord of the american lobster homarus americanus milne edwards, 1873 (malacostraca, pleocyemata, homarida). Dev Biol 258:44-56 (2003). Read more (PubMed: 12781681) »

    See all 11 Publications for this product

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample
    Fruit fly (Drosophila melanogaster) Cell (Drosophila Wing Imaginal disc)
    Specification
    Drosophila Wing Imaginal disc
    Permeabilization
    Yes - 0.5% Triton X100
    Fixative
    Paraformaldehyde
    Username

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    Submitted Mar 16 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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