Anti-Enhanced Blue Fluorescent Protein antibody [3A6] (ab32791)


  • Product nameAnti-Enhanced Blue Fluorescent Protein antibody [3A6]
  • Description
    Mouse monoclonal [3A6] to Enhanced Blue Fluorescent Protein
  • SpecificityCross reacts with GFP and YFP. Additional tests for cross reactivity have not been performed.
  • Tested applicationsSuitable for: ELISAmore details
  • Immunogen

    Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.


  • FormLyophilised:Dissolve the antibody in a Tris HCl pH 8.0 buffer, containing 0.05% sodium azide (dissolving 100 µg of antibody in 200 µl of buffer will result in a concentration of 0.5 mg/ml).
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 100mM Tris HCl. pH 8.0
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number3A6
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab32791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/1000F - 1/2000F.
    For the value of the multiplication factor F, see label on vial.
    Unless the stability in the actual test system has been established, it is
    recommended to dilute the product immediately before use.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceFluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems.

    References for Anti-Enhanced Blue Fluorescent Protein antibody [3A6] (ab32791)

    ab32791 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry free floating
    Sample Mouse Tissue sections (brain)
    Specification brain

    Abcam user community

    Verified customer

    Submitted Dec 19 2016

    Thank you for your reply.

    To our knowledge, ab32791 has not been tested in WB. Therefore, I can offer a discount off a future purchase if you buy ab32791now, test it inWB and submit feedback to us in the form of an Abreview. It doesn’t...

    Read More

    Thank you for contacting us.

    We have not tested ab1218 for reactivity with BFP. It is possible that this antibody may react with BFP, but as we have not mapped the epitope for this antibody, I do not have any further information about whether...

    Read More

    Thank you for contacting us.

    We are happy to help customers find the most suitable products for their research purposes; however there are many fluorescent proteins and we are not specialized in this particular field. We donot want to recomm...

    Read More

    Thank you for contacting us.

    We typically sell this antibody in lyophilized form to increase it's storage stability and shelf-life. We may be able to offer it in liquid form, but custom formulations are normally only available for bulk orders...

    Read More