Overview

  • Product name
  • Description
    Goat polyclonal to Enkephalin
  • Tested applications
    Suitable for: WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide:

    RSHHQDGSDNEE

    , corresponding to internal sequence amino acids 169-180 of Human Enkephalin

  • Positive control
    • Human Adrenal Gland lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa).
IHC-FoFr Use a concentration of 0.3 - 1 µg/ml.

Target

  • Function
    Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.
  • Sequence similarities
    Belongs to the opioid neuropeptide precursor family.
  • Post-translational
    modifications
    The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Enkephalin A antibody
    • Met-enkephalin-Arg-Phe antibody
    • OGF antibody
    • Opioid growth factor antibody
    • PENK antibody
    • PENK_HUMAN antibody
    • Preproenkephalin antibody
    • Proenkephalin A antibody
    • Proenkephalin antibody
    see all

Images

  • Anti-Enkephalin antibody (ab77273) at 0.3 µg/ml + Human Adrenal Gland lysate in RIPA buffer at 35 µg

    Predicted band size : 31 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of PFA-perfused frozen Human hypothalamus tissue, staining Enkephalin with ab77273 at 0.3 µg/ml.

References

ab77273 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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