The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa).
Use a concentration of 0.3 - 1 µg/ml.
FunctionMet- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.
Sequence similaritiesBelongs to the opioid neuropeptide precursor family.
Post-translational modificationsThe N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.