Anti-ENO1 antibody [253] - N-terminal (ab190365)
Key features and details
- Mouse monoclonal [253] to ENO1 - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-ENO1 antibody [253] - N-terminal
See all ENO1 primary antibodies -
Description
Mouse monoclonal [253] to ENO1 - N-terminal -
Host species
Mouse -
Specificity
ab190365 was tested for binding to expressed bovine ENO1, ENO2 and ENO3 and shown to be specific for only ENO1. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig -
Immunogen
Synthetic peptide corresponding to Cow ENO1 (N terminal). Immunogen corresponds to 12 amino acids synthesized on a 8-amine lysine core using the multiple antigen presentation method.
Database link: Q9XSJ4 -
Positive control
- HeLa cell lysate and rat 3T3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.07% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
253 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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ICC/IF |
1/2000 - 1/5000.
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Notes |
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WB
1/5000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
ICC/IF
1/2000 - 1/5000. |
Target
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Function
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. -
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
Sequence similarities
Belongs to the enolase family. -
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
Post-translational
modificationsISGylated. -
Cellular localization
Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line. - Information by UniProt
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Database links
- Entrez Gene: 2023 Human
- Entrez Gene: 100045967 Mouse
- Entrez Gene: 100503183 Mouse
- Entrez Gene: 13806 Mouse
- Entrez Gene: 433182 Mouse
- Entrez Gene: 24333 Rat
- Omim: 172430 Human
- SwissProt: P06733 Human
see all -
Alternative names
- 2 phospho D glycerate hydro lyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Alpha enolase antibody
see all
Images
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Immunofluorescence analysis of rat 3T3 cells, labeling ENO1 using ab190365 at a 1/2000 dilution (green). Counterstained with an antibody against Vimentin (red) and DNA (blue).
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Anti-ENO1 antibody [253] - N-terminal (ab190365) at 1/5000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 47 kDa
Observed band size: 47 kDa
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab190365 has been referenced in 3 publications.
- Huang T et al. Focal adhesion kinase-related non-kinase ameliorates liver fibrosis by inhibiting aerobic glycolysis via the FAK/Ras/c-myc/ENO1 pathway. World J Gastroenterol 28:123-139 (2022). PubMed: 35125823
- Czogalla B et al. Nuclear Enolase-1/ MBP-1 expression and its association with the Wnt signaling in epithelial ovarian cancer. Transl Oncol 14:100910 (2021). PubMed: 33069100
- Ye Y et al. Anti a-enolase antibody is a novel autoimmune biomarker for unexplained recurrent miscarriages. EBioMedicine 41:610-622 (2019). PubMed: 30827932