Recombinant
RabMAb

Anti-ENO1 antibody [EPR10864(B)] (ab155955)

Overview

  • Product name
    Anti-ENO1 antibody [EPR10864(B)]
    See all ENO1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10864(B)] to ENO1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human ENO1.

  • Positive control
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab155955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
      MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
    • Tissue specificity
      The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
    • Pathway
      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
    • Sequence similarities
      Belongs to the enolase family.
    • Developmental stage
      During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
    • Post-translational
      modifications
      ISGylated.
    • Cellular localization
      Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2 phospho D glycerate hydro lyase antibody
      • 2-phospho-D-glycerate hydro-lyase antibody
      • Alpha enolase antibody
      • Alpha enolase like 1 antibody
      • Alpha-enolase antibody
      • C myc promoter binding protein antibody
      • C-myc promoter-binding protein antibody
      • EC 4.2.1.11 antibody
      • eno1 antibody
      • ENO1L1 antibody
      • ENOA_HUMAN antibody
      • Enolase 1 (alpha) antibody
      • Enolase 1 (alpha) like 1 antibody
      • Enolase 1 antibody
      • Enolase alpha antibody
      • MBP 1 antibody
      • MBP-1 antibody
      • MBP1 antibody
      • MBPB1 antibody
      • MPB 1 antibody
      • MPB-1 antibody
      • MPB1 antibody
      • MYC promoter binding protein 1 antibody
      • NNE antibody
      • Non neural enolase antibody
      • Non-neural enolase antibody
      • Phosphopyruvate hydratase antibody
      • Plasminogen binding protein antibody
      • Plasminogen-binding protein antibody
      • PPH antibody
      • Tau crystallin antibody
      see all

    Images

    • All lanes : Anti-ENO1 antibody [EPR10864(B)] (ab155955) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 47 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ENO1 with ab155955 at 1/100 dilution.

    References

    ab155955 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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