Recombinant
RabMAb

Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892)

Overview

  • Product name
    Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422]
    See all ENO1 + ENO2 + ENO3 primary antibodies
  • Description
    Rabbit monoclonal [EPR18422] to ENO1 + ENO2 + ENO3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ENO1 + ENO2 + ENO3 aa 250 to the C-terminus. The exact sequence is proprietary. from ENO1.
    Database link: P06733

  • Positive control
    • WB: Human ENO1 full length recombinant protein, Human ENO2 full length recombinant protein, Human ENO3 full length recombinant protein, Human fetal liver lysate, Human fetal heart lysate, Human fetal kidney lysate, Human fetal spleen lysate, Mouse brain lysate, Mouse heart lysate, Mouse kidney lysate, Mouse spleen lysate, Rat brain lysate, Rat heart lysate, Rat spleen lysate, HeLa, Jurkat MCF7 , A431, C6, Raw264.7 and NIH/3T3 whole cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab189892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

Target

  • Relevance
    Enolase 1 is a multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. Enolase 2 has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. Enolase 3 appears to have a function in striated muscle development and regeneration.
  • Cellular localization
    ENO1: Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M-band. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M-band. ENO2: Cytoplasm. Cell membrane. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form ENO3: Cytoplasm. Note: Localized to the Z line. Some colocalization with CKM at M-band.
  • Database links
  • Alternative names
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Alpha-enolase antibody
    • Beta-enolase antibody
    • C-myc promoter-binding protein antibody
    • Crystallin Tau, included antibody
    • ENO1L1 antibody
    • Enolase 1, (alpha) antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 3 (beta, muscle) antibody
    • Enolase 3 antibody
    • Enolase alpha antibody
    • Enolase, beta antibody
    • Enolase, gamma antibody
    • Enolase, muscle specific antibody
    • Enolase, neuron specific antibody
    • Enolase, nonneuronal, included antibody
    • Gamma-enolase antibody
    • GSD13 antibody
    • MBP-1 antibody
    • MPB1 antibody
    • MSE antibody
    • Muscle-specific enolase antibody
    • Neural enolase antibody
    • Neuron-specific enolase antibody
    • NNE antibody
    • Non-neural enolase antibody
    • NSE antibody
    • Phosphpyruvate hydratase antibody
    • Plasminogen-binding protein antibody
    • Skeletal muscle enolase antibody
    see all

Images

  • All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892) at 1/5000 dilution

    Lane 1 : Human ENO1 full length recombinant protein
    Lane 2 : Human ENO2 full length recombinant protein
    Lane 3 : Human ENO3 full length recombinant protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa


    Exposure time: 5 seconds


    Blocking and diluting buffer 5% NFDM /TBST.

    Human ENO1 full length recombinant protein (Cat#:ab89248) containing aa1-434.

    Human ENO2 full length recombinant protein containing aa1-434 with a His-Tag®. 

    Human ENO3 full length recombinant protein (Cat#:ab113127) containing aa1-434 with a His-Tag®.Human ENO2 full length recombinant protein was made in-house.

  • All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892) at 1/5000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa


    Exposure time: 5 seconds


    Blocking and diluting buffer 5% NFDM /TBST.

    Exposure time - Lane 1,2 and 3:5 seconds;
    Lane 4: 30 seconds

  • All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 4 : A431 (Human epidermoid carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa


    Exposure time: 3 seconds


    Blocking and diluting buffer was 5% NFDM /TBST.

  • Lanes 1-7 : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892) at 1/5000 dilution
    Lanes 8-10 : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate
    Lane 7 : Rat spleen lysate
    Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 9 : Raw264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 10 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa


    Exposure time: 1 second


    Blocking and diluting buffer was 5% NFDM /TBST.

    Exposure time -  Lane 1-4 and 8-10: 1 second;
    Lane 5-7: 3 seconds;

References

ab189892 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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