Overview

  • Product name
  • Description
    Rabbit polyclonal to ENO3
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-371 of Human ENO3.

  • Positive control
    • A549, HeLa, HepG and HCT116 whole cell lysates; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab126259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 47 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Appears to have a function in striated muscle development and regeneration.
  • Tissue specificity
    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Involvement in disease
    Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13) [MIM:612932]. A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen-beta particles are detected on ultrastructural analysis.
  • Sequence similarities
    Belongs to the enolase family.
  • Developmental stage
    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Cellular localization
    Cytoplasm. Localized to the Z line. Some colocalization with CKM at M-band.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2 phospho D glycerate hydrol yase antibody
    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Beta enolase antibody
    • Beta-enolase antibody
    • ENO3 antibody
    • ENOB_HUMAN antibody
    • Enolase 3 (beta muscle) antibody
    • Enolase 3 antibody
    • Enolase3 antibody
    • GSD13 antibody
    • MSE antibody
    • Muscle specific enolase antibody
    • Muscle-specific enolase antibody
    • Skeletal muscle enolase antibody
    see all

Images

  • Anti-ENO3 antibody (ab126259) at 1/10000 dilution + HepG2 cell lysate at 30 µg

    Predicted band size : 47 kDa
  • ab126259 at 1/500 dilution staining ENO3 in methanol-fixed HeLa cells by Immunofluorescence (green). Alpha tubulin filaments are stained red.

References

ab126259 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Tissue lysate - whole (skeletal muscle)
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Rafael Montes Nieto

Verified customer

Submitted Oct 31 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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