Overview

  • Product nameAnti-ENO3 antibody
    See all ENO3 primary antibodies
  • Description
    Rabbit polyclonal to ENO3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 196-413 of Human ENO3 (AAH17249).

  • Positive control
    • HepG2, Molt-4 and Raji whole cell lysates; 293T, A431, H1299 and HeLa cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab96334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.

Target

  • FunctionAppears to have a function in striated muscle development and regeneration.
  • Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Involvement in diseaseDefects in ENO3 are the cause of glycogen storage disease type 13 (GSD13) [MIM:612932]. A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen-beta particles are detected on ultrastructural analysis.
  • Sequence similaritiesBelongs to the enolase family.
  • Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Cellular localizationCytoplasm. Localized to the Z line. Some colocalization with CKM at M-band.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2 phospho D glycerate hydrol yase antibody
    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Beta enolase antibody
    • Beta-enolase antibody
    • ENO3 antibody
    • ENOB_HUMAN antibody
    • Enolase 3 (beta muscle) antibody
    • Enolase 3 antibody
    • Enolase3 antibody
    • GSD13 antibody
    • MSE antibody
    • Muscle specific enolase antibody
    • Muscle-specific enolase antibody
    • Skeletal muscle enolase antibody
    see all

Anti-ENO3 antibody images

  • All lanes : Anti-ENO3 antibody (ab96334) at 1/1000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : Molt-4 whole cell lysate
    Lane 3 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 47 kDa

References for Anti-ENO3 antibody (ab96334)

ab96334 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (hindpaw skin)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification hindpaw skin
Blocking step LiCOR blocking buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
Username

Dr. maral tajerian

Verified customer

Submitted Aug 26 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"