Recombinant
RabMAb

Anti-ENO3 antibody [EPR11366(B)] (ab157474)

Overview

  • Product name
    Anti-ENO3 antibody [EPR11366(B)]
    See all ENO3 primary antibodies
  • Description
    Rabbit monoclonal [EPR11366(B)] to ENO3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ENO3 aa 400-500. The exact sequence is proprietary.

  • Positive control
    • HeLa, HepG2, Human spleen, fetal heart and fetal muscle lysates; Immunoprecipitation pellet from fetal muscle lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 47 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Appears to have a function in striated muscle development and regeneration.
    • Tissue specificity
      The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
    • Pathway
      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
    • Involvement in disease
      Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13) [MIM:612932]. A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen-beta particles are detected on ultrastructural analysis.
    • Sequence similarities
      Belongs to the enolase family.
    • Developmental stage
      During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
    • Cellular localization
      Cytoplasm. Localized to the Z line. Some colocalization with CKM at M-band.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2 phospho D glycerate hydrol yase antibody
      • 2 phospho D glycerate hydrolyase antibody
      • 2-phospho-D-glycerate hydro-lyase antibody
      • Beta enolase antibody
      • Beta-enolase antibody
      • ENO3 antibody
      • ENOB_HUMAN antibody
      • Enolase 3 (beta muscle) antibody
      • Enolase 3 antibody
      • Enolase3 antibody
      • GSD13 antibody
      • MSE antibody
      • Muscle specific enolase antibody
      • Muscle-specific enolase antibody
      • Skeletal muscle enolase antibody
      see all

    Images

    • All lanes : Anti-ENO3 antibody [EPR11366(B)] (ab157474) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Human spleen lysate
      Lane 4 : Fetal heart lysate
      Lane 5 : Fetal muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 47 kDa

    • Anti-ENO3 antibody [EPR11366(B)] (ab157474) at 1/1000 dilution + immunoprecipitation pellet from fetal muscle lysate

      Secondary
      HRP-conjugated anti-rabbit IgG, which
      preferentially detects the non-reduced form of
      rabbit IgG

      Predicted band size: 47 kDa



      Note: primary antibody diluted with 1% BSA.

    References

    ab157474 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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